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Team:Warsaw/Calendar-Main/23 July 2008
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<ol><li>Results of ligation: 6 colonies grown.</li> | <ol><li>Results of ligation: 6 colonies grown.</li> | ||
<li>Each colony cultured overnight in LB + ampicillin.</li></ol> | <li>Each colony cultured overnight in LB + ampicillin.</li></ol> | ||
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<h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4> | <h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4> | ||
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_A_omega).</li> | <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_A_omega).</li> | ||
| - | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer). </li></ol> | + | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer). Proper clone founded. </li></ol> |
<h4>Antoni</h4> | <h4>Antoni</h4> | ||
Revision as of 19:43, 11 October 2008
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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
Cloning of protein A DNA to OmpA constructsMichał K.
AntoniSetup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria.
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