Team:Warsaw/Calendar-Main/23 July 2008
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- | + | <h3>Cloning omega-A fusion on pKS (second attempt)</h3> | |
+ | <h4>Michał L., Ewa, Marcin</h4> | ||
+ | <ol> | ||
+ | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from transformants.</li> | ||
+ | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>Digest</a> of plasmids with SacI and NotI (BamHI buffer).</li> | ||
+ | <li>Gel electrophoresis of products of digest.</li> | ||
+ | <li>2 selected clones were send to DNA sequencing lab.</li> | ||
+ | </ol> | ||
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4> | <h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4> |
Revision as of 20:12, 11 October 2008
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
Cloning of protein A DNA to OmpA constructsMichał K.
AntoniSetup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria.
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