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Alberta NINT/17 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/16_July_2008 | < Previous entry]] | + | [[Alberta_NINT/16_July_2008 | < Previous entry]] | [[Alberta_NINT/18_July_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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K102032+K102036 (LB+amp50+kan35) | K102032+K102036 (LB+amp50+kan35) | ||
| - | + | lab work (JD): | |
| + | Gel purification of pSB4A5/E+P, K102003/E+P, K102006/E+P | ||
| + | Quantitated the samples | ||
| + | Carried out ligations: K102003+pSB4A5=K102031, and K102006+pSB4A5=K102021 | ||
| + | Transformed XL1-B Cells | ||
| + | Plated cells on LB+Amp plates | ||
Latest revision as of 23:00, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Since the double-plasmid cell cultures did grow on the LB+kan35 plates we will try again to
grow them on LB+amp+kan35. This time, 2.0ul of each of the plasmids is transformed into XL1-B
cells and plated in an attempt to increase transformation efficiency.
K102032+K102011 (LB+amp50+kan35)
K102032+K102034 (LB+amp50+kan35)
K102032+K102035 (LB+amp50+kan35)
K102032+K102036 (LB+amp50+kan35)
lab work (JD):
Gel purification of pSB4A5/E+P, K102003/E+P, K102006/E+P
Quantitated the samples
Carried out ligations: K102003+pSB4A5=K102031, and K102006+pSB4A5=K102021
Transformed XL1-B Cells
Plated cells on LB+Amp plates
