Alberta NINT/22 July 2008

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lab work (SD):
lab work (SD):
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             Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and  
             Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and  
             plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35.
             plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35.
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Latest revision as of 23:01, 11 October 2008

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lab work (SD):

            Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells.
            Growth visible in K102022 and XL1-B wells.  Put 96-well plate back at 37 C.
            Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and 
            plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35.