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Alberta NINT/29 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/28_July_2008 | < Previous entry]] | + | [[Alberta_NINT/28_July_2008 | < Previous entry]] | [[Alberta_NINT/30_July_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P). | Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P). | ||
| - | + | lab work (JD): | |
| - | + | Began creating new input plasmids | |
| + | Anneal oligos: TAIn_2, TAIn_3, TAIn_6, TAIn_7 | ||
| + | Digest oligos with XhoI and HindIII | ||
| + | Digest K102011 with XhoI and HindIII | ||
| + | Nucleotide removal kit for TAIn_X/XhoI and HindIII | ||
| + | PCR purification kit for K102011/XhoI and HindIII | ||
Latest revision as of 23:04, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Isolated DNA (K102031 and K102032) from 28/07/08 LB + amp50 culture tubes using QIAprep spin
miniprep protocol, using 5 ml of culture and a minelute spin column, eluting in 15 ul of Buffer EB
to maximize the concentration of DNA obtained.
Digested K102032 and K102031 with EcoRI + PstI.
Ran on 2% agarose gel (110V 50 minutes) and excised the 50 bp fragment from K102032 and the 3 kb
fragment from K102031.
Extracted DNA from gel usig QIAquick gel extraction protocol.
Ligated K102032/E+P with pSB4A5 (from K102031 /E+P)(Negative control K102031 /E+P).
lab work (JD):
Began creating new input plasmids
Anneal oligos: TAIn_2, TAIn_3, TAIn_6, TAIn_7
Digest oligos with XhoI and HindIII
Digest K102011 with XhoI and HindIII
Nucleotide removal kit for TAIn_X/XhoI and HindIII
PCR purification kit for K102011/XhoI and HindIII
