"
Alberta NINT/31 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: < Back to notebook < Previous entry lab work (JD): Counted colonies on both mine and Shannon's plates from ...) |
m |
||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/30_July_2008 | < Previous entry]] | + | [[Alberta_NINT/30_July_2008 | < Previous entry]] | [[Alberta_NINT/1_August_2008 | Next entry >]] |
| + | |||
lab work (JD): | lab work (JD): | ||
Counted colonies on both mine and Shannon's plates from yesterday's ligations | Counted colonies on both mine and Shannon's plates from yesterday's ligations | ||
| - | -unfortunately equal numbers on negative plates and ligation plates so ligation did not work | + | -unfortunately equal numbers on negative plates and ligation plates so ligation |
| - | -my plate had 100s on the negative control so I assumed the vector was not cut properly | + | did not work |
| + | -my plate had 100s on the negative control so I assumed the vector was not cut | ||
| + | properly | ||
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel | ||
Latest revision as of 23:05, 11 October 2008
< Previous entry | Next entry >
lab work (JD):
Counted colonies on both mine and Shannon's plates from yesterday's ligations
-unfortunately equal numbers on negative plates and ligation plates so ligation
did not work
-my plate had 100s on the negative control so I assumed the vector was not cut
properly
Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel
