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Team:Warsaw/Calendar-Main/22 May 2008
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li> | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li> | ||
| - | <li> Gel electrophoresis (no proper colonies found). Fig. 1.</li> | + | <li> Gel electrophoresis (no proper colonies found). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008#fig1">Fig. 1</a>.</li> |
| - | <img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/> | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/8/87/Aid_fusion_digests_WAW.jpg"width=300/></a> |
<var><b>Fig. 1.</b> 1-DNA ladder;<br> | <var><b>Fig. 1.</b> 1-DNA ladder;<br> | ||
2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); <br> | 2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); <br> | ||
Revision as of 23:11, 11 October 2008
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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Piotr
Design of primers for sequencing something we want from pMPM-T5. Michał K.
Fig. 1. 1-DNA ladder;2 and 3-hypothetical pMPM-T5 with transcription fusion digested with EcoRI (2 clones); 4-pMPM-T5-AID digested with EcoRI; 5-hypothetical pMPM with translation fusion digested with BamHI; 6-pMPM-T5-AID digested with BamHI. |
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