Team:Warsaw/Calendar-Main/21 August 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4>
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<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:
<li> Colony PCR on colonies from plates with transformations pGeneart+A Primers used:

Revision as of 16:16, 12 October 2008

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Cloning of protein A DNA to GeneArt plasmid

Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin
  3. Inoculation to liquid LB with ampicillin: pET15b+OmpA-alfa

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