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Team:Warsaw/Calendar-Main/21 August 2008
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<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4> | <h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4> | ||
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| - | <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pGeneart+A. <br>Primers used: | + | <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a>. <br>Primers used: |
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li> | ||
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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin. | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin. | ||
</li> | </li> | ||
| - | <li>Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.</li> | + | <li>Inoculation to liquid LB with ampicillin: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a>.</li> |
</ol></p> | </ol></p> | ||
| - | <h3>Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega</h3><h4>Michał K.</h4> | + | <h3>Cloning of alpha to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a></h3><h4>Michał K.</h4> |
<p><ol> | <p><ol> | ||
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day. </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day. </li> | ||
Revision as of 11:16, 19 October 2008
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Cloning of protein A DNA to GeneArt plasmidAntoni
Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
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