OmpA_A_alpha, OmpA_Adelta_alpha, OmpA_Adelta_omega, OmpA_A_omega, OmpA_omega_Adelta, OmpA_omeg

<tr><th>Ompa-Z-alpha</th><td>His+A+omega</td><td>yes</td><td>no</td></tr> <tr><th>Ompa-Z-alpha</th><td>His+A+omega</td><td>yes</td><td>no</td></tr> </table>

Cloning of protein A DNA to GeneArt plasmid

Antoni

1. Colony PCR on colonies from plates with transformations pGeneart+A.
   Primers used: AP+NotI and AL+SacI.
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
4. Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with FastBamHI and FastAcc65I (Fast Digest buffer).
3. Gel electrophoresis - no proper clones founded.

OmpA variant ("hunter")	"Prey" variant	Growth with prey	Growth without prey
OmpA-alpha	His+Z+omega	no	no
OmpA-alpha	His+Z+alfa	no	no
OmpA-A-alpha	His+Z+omega	yes	no
OmpA-Z-omega	His+A+alpha	yes	no
OmpA-omega-A-alpha	His+Z+omega	yes	no
Ompa-A-omega	His+Z+alpha	yes	no
Ompa-A-omega	His+Z+alpha	yes	no
OmpA-omega	His+A+alpha	no</th><td>no