BioBricks
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| comment
| Date
|
MG1655
| Strain
| S141
|
| Strain
|
| Aug 7
|
[http://partsregistry.org/Part:pSB4T5 pSB4T5]
| [http://partsregistry.org/Part:pSB4T5 pSB4T5]
| S215
| MP215
| plasmid
| low copy plasmid with pSC101 ORI
| October 14
|
[http://partsregistry.org/Part:pSB1A3 pSB1A3]
| [http://partsregistry.org/Part:pSB1A3 pSB1A3]
| S122
| MP115
| plasmid
|
| July 23
|
[http://partsregistry.org/Part:BBa_J61002 J61002]
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S142
| MP123
| plasmid
|
| August 5
|
[http://partsregistry.org/Part:pSB3K3 pSB3K3]
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S158
| x
| plasmid
|
| August 15
|
[http://partsregistry.org/Part:pSB3K3 pSB3K3]
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| x
| MP142
| plasmid
| low to medium copy plasmid with p15A ORI
| August 8
|
[http://partsregistry.org/Part:BBa_I0500 I0500]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| S103
| MP119
| AraC pBAD
|
| July 24, July 26
|
[http://partsregistry.org/Part:BBa_J23100 J23100]
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| x
| MP116
| Strong constitutive promoter
|
| July 26
|
[http://partsregistry.org/Part:BBa_J23107 J23107]
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| x
| MP117
| Medium constitutive promoter
|
| July 24, July 26
|
L135
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
|
|
| pFlgA
| D137 (BV) - D133 (BI) don't work
| August 9
|
L136
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
|
|
| pFlgB
| D137 (BV) - D1343 (BI) don't work
| August 9
|
L137
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
|
|
| pFlhB
| D137 (BV) - D135 (BI) don't work
| August 9
|
L143
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| x
| x
| pFliL
| D149 - D137 didn't work
| August 14
|
L144
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| x
| x
| pFliL
| D150 - D152 didn't work
| August 14
|
L147
| [http://partsregistry.org/Part:J61002 J61002]
| x
| x
| pFlhDC
| D155 - D136 didn't work
| August 16
|
L
| [http://partsregistry.org/Part:J61002 J61002]
| S207
| MP207
| pFliA
|
| October 04
|
[http://partsregistry.org/Part:BBa_J23101 J23101]
| [http://partsregistry.org/Part:BBa_J61002 J61002 ]
| S109
| MP101
| Strongest Constitutive promoter
|
| July 22, July 23, July 26
|
[http://partsregistry.org/Part:BBa_J23109 J23109]
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S110
| MP102
| Weak Constitutive promoter
|
| July 22, July 26
|
[http://partsregistry.org/Part:BBa_R0079 R0079]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S108
| MP103
| promoter pLas (lasr & PAI regulated)
|
| July 23, July 26
|
[http://partsregistry.org/Part:BBa_R0040 R0040]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S111
| MP104
| tetR repressible promoter
|
| July 23, July 26
|
pFlhB
| pCR4Blunt-TOPO
| x
| MP175
| pFlhB-RBS (WT)
|
| September 04
|
pFliA
| pCR4Blunt-TOPO
| x
| MP176
| pFliA-RBS (WT)
| with conserved Pst1 site
| September 04
|
pFlhDC
| pCR4Blunt-TOPO
| x
| MP177
| pFlhDC-RBS (WT)
|
| September 04
|
pFliL
| pCR4Blunt-TOPO
| x
| MP178
| pFliL-RBS (WT)
|
| September 04
|
pFlgB
| pCR4Blunt-TOPO
| S187
| MP184
| pFlgB-RBS (WT)
|
| September 04
|
L
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S210
| MP210
| pFliL(RBS WT)
|
| October 10
|
L
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S211
| MP211
| pFlgA(RBS WT)
|
| October 10
|
L
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S212
| MP212
| pFlhB(RBS WT)
|
| October 10
|
L
| pCR4Blunt-TOPO
| S179
| MP176
| pFliA-RBS (WT)
| pFliA with Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S180
| MP177
| pFlhDC-RBS (WT)
|
| September 04
|
L
| pCR4Blunt-TOPO
| S181
| MP178
| pFliL-RBS (WT)
|
| September 04
|
[http://partsregistry.org/Part:BBa_B0034 B0034 ]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S107
| MP100
| Strongest RBS (Efficiency = 1)
|
| July 26
|
[http://partsregistry.org/Part:BBa_B0032 B0032]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S163
| MP163
| medium rbs (efficiency = 0,3)
|
| August 20
|
[http://partsregistry.org/Part:BBa_B0030 B0030]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| MP120
| Strong RBS (Efficiency = 0,6)
|
| July 26
|
[http://partsregistry.org/Part:BBa_S03154 S03154]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S112
| MP105
| B0034(rbs) ->LasI
|
| July 26
|
[http://partsregistry.org/Part:BBa_S03879 S03879]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S113
| MP106
| B0034(rbs) -> TetR
|
| August 24
|
L
| pCR4Blunt-TOPO
| S182
| MP179
| RBS(FlhDC) - FlhDC
| FlhDC with conserved Pst1 site
| September 04
|
[http://partsregistry.org/Part:BBa_C0079 C0079]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| S114
| MP107
| lasR activator with LVA Tag
|
| July 23
|
[http://partsregistry.org/Part:BBa_C0179 C0179]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S115
| MP108
| lasR activator
|
| July 22, July 23, July 26
|
OmpR M57I
|
| S119
| MP112
| OmpR
|
| July 23
|
EnvZ I281S
|
| S120
| MP113
| EnvZ
|
| July 23
|
[http://partsregistry.org/Part:BBa_C0040 C0040]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S121
| MP114
| tetR repressor
|
| July 23
|
FliA2
| pCR4Blunt-TOPO
| S183
| MP180
| FliA2
| mutated at EcoR1 and Pst1 internal site
| September 04
|
FliA3
| pCR4Blunt-TOPO
| x
| MP181
| FliA3
| mutated at EcoR1 and Pst1 site
| September 04
|
EnvZ*
| pCR4Blunt-TOPO
| x
| MP182
| EnvZ*
| the good one but not mutated for EcoR1 site
| September 04
|
OmpR WT
| pCR4Blunt-TOPO
| x
| MP183
| OmpR WT
| not the mutated
| September 04
|
OmpR WT
|
| x
| MP185
| OmpR WT
| not the mutated
| September 04
|
L132
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S153
| MP154
| flhDC
| D142(FV) - D139(FI)
| August 13
|
L133
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S154
| MP155
| OmpR*
| D142(FV) - D140(FI) WT sequence
| August 19
|
L134
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S155
| MP156
| EnvZ*
| D142(FV) - D141(FI) lacks about 300 bp in EnvZ*
| August 19
|
L140
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
|
|
| flhD
| D145 (V) - D141 (I)
| August 12
|
L141
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S160
| MP159
| flhC
| D142 (ES) - D145 (ES) has not been sequenced
| August 15
|
L145
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| x
| FlhDC
| D1543 - D145 didn't work
| August 16
|
L146
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| x
| FlhDC
| D154 - D145 didn't work
| August 16
|
L
| pCR4Blunt-TOPO
| S179
| MP180
| FliA
| mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S184
| MP181
| FliA3
| FliA mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S185
| MP182
| EnvZ*
| the good one but not mutated for EcoR1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S186
| MP183
| OmpR WT
| OmpR WT not the mutated
| September 04
|
L
| pCR4Blunt-TOPO
| S188
| MP185
| OmpR WT
| OmpR WT not the mutated
| September 04
|
[http://partsregistry.org/Part:BBa_E0030 E0030]
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| S116
| MP109
| YFP
|
| July 26
|
[http://partsregistry.org/Part:BBa_E0040 E0040]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S117
| MP110
| GFP
|
| July 23, July 26
|
[http://partsregistry.org/Part:BBa_E1010 E1010 ]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| S118
| MP111
| mRFP
|
| July 23, July 26
|
[http://partsregistry.org/Part:BBa_E0422 E0422]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S164
| MP121
| ECFP (RBS+LVA+Double Term)
|
| July 24, July 26
|
[http://partsregistry.org/Part:BBa_E0840 E0840]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S106
| MP122
| gfp tri-part; strong rbs
|
| July 26
|
[http://partsregistry.org/Part:BBa_E0430 E0430]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S165
| MP165
| RBS+ YFP+ LVA- term
|
| August 20
|
[http://partsregistry.org/Part:BBa_E0432 E0432]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S166
| MP166
| RBS+ YFP LVA+ term
| BUT be careful , it doesn't work. See registry
| August 20
|
[http://partsregistry.org/Part:BBa_E0420 E0420]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S167
| MP167
| RBS+ ECFP LVA- term
|
| August 20
|
[http://partsregistry.org/Part:BBa_I732078 I732078]
| [http://partsregistry.org/Part:pSB1A3 pSB1A3]
| S168
| MP168
| RBS+ mRFP LVA+ term
|
| August 20
|
[http://partsregistry.org/Part:BBa_E0240 E0240]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S157
| x
| GFP generator
|
| August 15
|
[http://partsregistry.org/Part:BBa_E0434 E0434]
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| S216
| x
| YFP tripart +AAV
|
| October 15
|
[http://partsregistry.org/Part:BBa_E0240 E0240]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| MP143
| gfp generator
|
| August 8
|
[http://partsregistry.org/Part:BBa_E0422 E0422]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| MP164
| RBS+ ECFP+ LVA+ term
|
| August 20
|
[http://partsregistry.org/Part:BBa_B0015 B0015]
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| x
| MP118
| Double terminator
|
| July 24, July 26
|
Ligations: Constructions realised
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| Digestion
| comment
| Date
|
L100
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S146
| MP147
| rbs TetR - ECFP
| D110 (BV) - D130 (BI)
|
| August 13
|
L101
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S147
| MP148
| rbs TetR - GFP tripart
| D110 (BV) - D131 (BI)
| DO NOT WORK !!!!
| August 13
|
L102
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S123
| MP124
| Strong rbs - YFP
| D129 (BV) - D118 (BI)
|
| August 1
|
L103
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S124
| MP125
| Strong rbs - mRFP
| D129 (BV) - D122 (BI)
|
| August 1
|
L104
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S125
| MP126
| Strong rbs - lasR activator
| D129 (BV) - D114 (BI)
|
| August 1
|
L105
| [http://partsregistry.org/Part:J61002 J61002]
| S126
| MP127
| Strong promoter - ECFP
| D123 (BV) - D130 (BI)
|
| August 1
|
L106
| [http://partsregistry.org/Part:J61002 J61002]
| S127
| MP128
| Strong promoter - gfp Tripart
| D123 (BV) - D131 (BI)
|
| August 1
|
L107
| [http://partsregistry.org/Part:J61002 J61002]
| S128
| MP129
| Strong promoter - ECFP
| D103 (BV) - D130 (BI)
|
| August 1
|
L108
| [http://partsregistry.org/Part:J61002 J61002]
| S129
| MP130
| Strong promoter - gfp Tripart
| D103 (BV) - D131 (BI)
|
| August 1
|
L109
|
|
|
|
|
|
| August
|
L110
| [http://partsregistry.org/Part:J61002 J61002]
| S130
| MP131
| Medium promoter - gfp Tripart
| D124 (BV) - D131 (BI)
|
| August 1
|
L111
| [http://partsregistry.org/Part:J61002 J61002]
| S131
| MP132
| Weak promoter - ECFP
| D104 (BV) - D130 (BI)
|
| August 1
|
L112
| [http://partsregistry.org/Part:J61002 J61002]
| S132
| MP133
| Weak promoter - GFP tripart
| D104 (BV) - D131 (BI)
|
| August 1
|
L113
|
|
|
|
|
|
| August
|
L114
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| S148
| MP149
| AracpBAD - gfp tripart
| D126 (BV) - D131 (BI)
|
| August 13
|
L115
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S133
| MP134
| pLas - ECFP
| D105 (BV) - D130 (BI)
|
| August 1
|
L116
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S134
| MP135
| pLas - gfp Tripart
| D105 (BV) - D131 (BI)
|
| August 1
|
L117
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| S135
| MP136
| yfp - Double Terminator
| D125 (FV) - D117 (FI)
|
| August 1
|
L118
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| S136
| MP137
| rfp - Double Terminator
| D125 (FV) - D121 (FI)
|
| August 1
|
L119
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| S137
| MP138
| lasR activator + LVA - Double Terminator
| D125 (FV) - D113 (FI)
|
| August 1
|
L120
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S149
| MP150
| tetR repressible promoter - ECFP
| D106 (BV) - D130 (BI)
|
| August 13
|
L121
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S138
| MP139
| pTet - gfp tripart
| D106 (BV) - D131 (BI) In Top10 strain
|
| August 1
|
L121
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S171
| not done
| pTet - gfp Tripart
| D106 (BV) - D131 (BI)
| Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population.
| Aug 22
|
L122
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S150
| MP151
| RBS-lasI - ECFP
| D107 (BV) - D130 (BI)
|
| August 13
|
L123
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S151
| MP152
| RBS lasI - gfp tripart
| D107 (BV) - D131 (BI)
|
| August 13
|
L124
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S139
| MP140
| Strongest RBS - rfp
| D102 (BV) - D122 (BI)
|
| August 1
|
L125
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S140
| MP141
| Strongest RBS - yfp
| D102 (BV) - D118 (BI)
|
| August 1
|
L126
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S152
| MP153
| Strongest RBS (1)- LasR activator (+LVA)
| D102 (BV) - D114 (BI)
|
| August 13
|
L128
| [http://partsregistry.org/Part:J61002 J61002]
| S143
| MP144
| pFlgA-RFP
| D132 (FI) - D136 (FV)
|
| August 12
|
L129
| [http://partsregistry.org/Part:J61002 J61002]
| S144
| MP145
| pFlgB-RFP
| D133 (FI) - D136 (FV)
|
| August 12
|
L130
| [http://partsregistry.org/Part:J61002 J61002]
| S145
| MP146
| pFlhB-RFP
| D134 (FI) - D136 (FV)
| wrong sequencing
| August 12
|
L131
| [http://partsregistry.org/Part:J61002 J61002]
|
|
| pFlhDC-RFP
| D136 (FV)- D135 (FI)
| wrong sequencing
| August 8
|
|
L138
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S156
| MP157
| gfp generator (E0240)
| D137(FV) - D138(FI)
|
| August 13
|
L139
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S159
| MP158
| E0240 RBS+
| D140 (PstI) - D144 (ES)
| seems to have the plasmid without the insert and one with the insert
| August 15
|
L142
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S161
| MP160
| flhDC+promotor
| D143 (ES) - D145 (ES)
| does not have the insert
| August 15
|
L148
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| x
| rbs (B0034)- OmpR*
| D158 - D102
| didn't work
| August 16
|
L149
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| x
| rbs (B0034)- EnvZ
| D159 - D102
| didn't work
| August 16
|
L150
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| x
| MP161
| pLas + rbs-tetR-gfp tripart
| D105 (BV) - D146 (BI)
| incorrect clone -> to do again
| August 19
|
L151
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| S162
| MP162
| rbs-lasR + double terminator
| D147 (FI) - D125 (FV)
|
| August 19
|
L153
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S169
| MP169
| pJ23100-rbs-lasER-dble ter
| D123 (BV) - D165 (BI)
|
| August 20
|
L154
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S170
| MP170
| pJ23101-rbs-lasER-dble ter
| D103 (BV) - D165 (BI)
|
| August 20
|
L155
|
|
|
| pJ23101-gfp generator
| D164(FV) - D163 (FI)
|
| August 22
|
L156
|
|
|
| pTet-gfp generator
| D161 (FV) - D163 (FI)
|
| August 22
|
L157
|
|
|
| tetR-rbs (B0015)
| D125 (BV) - D162 (BI)
|
| August 23
|
L158
|
| x
| x
| rbs-tetR-gfp generator
| D110(BV) - D131 (BI)
| didn't work
| August 23
|
L159
|
| x
| x
| rbs-lasI-dbl ter
| D125 (FV) - D109 (FI)
| didn't work
| August
|
L160
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S174
| MP173
| pFlgA - YFP Tripart (LVA-)
| D166 (FV) - D132 (FI)
| Fluorescence OK
| August 22
|
L161
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S175
| MP174
| pFlgA - YFP Tripart (LVA+)
| D167 (FV) - D132 (FI)
|
| August 22
|
L162
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| x
| x
| rbs-TetR-gfp generator
| D110 (BV) - D163 (BI)
|
| August 22
|
L163
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| x
| x
| rbs-lasI-gfp generator
| D107 (BV) - D163 (BI)
|
| August 22
|
L166
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S173
| MP172
| RBS(B0032) - tetR
|
|
| August 25
|
L167
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| S172
| MP171
| gfp generator(E0240) - pTet
|
|
| August 25
|
L174
| [http://partsregistry.org/Part:J61002 J61002]
| S
| MP202
|
Strong constitutive promoter-GFP generator
| J23101-E0240
|
| September 26
|
L176
|
|
|
|
| D125 (v) + D162 (i)
|
| September
|
L179
| [http://partsregistry.org/Part:J61002 J61002]
| S200
| MP200
| J23101 + RBS (B0032) + tet R
|
|
| September 24
|
L180
|
|
|
|
| D166 (v) + D109.2 (i)
|
| September
|
L181
|
|
|
|
| D199 (v) + D109.2 (i)
|
| September
|
L182
|
|
|
|
| D187 (v) + D109.2 (i)
|
| September
|
L186
|
|
|
|
| D179 (v) + D205 (i)
|
| September
|
L190 ("L4")
|
|
|
|
| D199 (v) + D (i)
|
| September
|
L195 ("L9")
|
|
|
|
| D187 (v) + D194 (i)
|
| September
|
L ([http://partsregistry.org/Part:BBa_J23100 J23100]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015])
| [http://partsregistry.org/Part:J61002 J61002]
| S169
| MP169
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
L ([http://partsregistry.org/Part:BBa_J23101 J23101]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015])
| [http://partsregistry.org/Part:J61002 J61002]
| S170
| MP170
| strong constitutive promoter-RBS-LasR-term
|
|
| August 21
|
L
| pCR4Blunt-TOPO
| S178
| MP175
| pFlhB-RBS (WT)
|
|
| September 04
|
L
| pCR4Blunt-TOPO
| S
| MP184
| pFlgB-RBS (WT)
|
|
| September 04
|
L
| [http://partsregistry.org/Part:J61002 J61002]
| S201
| MP201
| pFlgA(RBS WT)-EnvZ*
|
|
| September 24
|
L
| [http://partsregistry.org/Part:pSB3K3 pSB3K3]
| S208
| MP208
| pFlgA(RBS WT)-EnvZ*
|
| without pFlgA indeed
| October 04
|
L
| [http://partsregistry.org/Part:J61002 J61002]
| S209
| MP209
| pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+
|
| without pFlgA indeed
| October 04
|
L
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S213
| MP213
| pFlhB(RBS WT)-mRFP
pFlhB(RBS WT)-mRFP
|
|
| October 10
|
L
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| S214
| MP214
| pFliL(RBS WT)-mRFP
|
|
| October 10
|
RBS WT-FlhDC
| pCR4Blunt-TOPO
| x
| MP179
| RBS(FlhDC) - FlhDC
| with conserved Pst1 site
| September 04
|
Digestion Products
|
Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Obs
| Size Matrix (bp)
| Size BB (bp)
| Description
| Product in use
| []ng/µL
| Date
|
D100.1
| [http://partsregistry.org/Part:BBa_B0034 B0034]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 34
|
|
| 10
| July 26
|
D100.2
|
D101
| [http://partsregistry.org/Part:BBa_B0034 B0034]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
| 2076
| 15
|
|
| 10
| July 29
|
D102.1
| [http://partsregistry.org/Part:BBa_B0034 B0034]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| 2077
| 14
|
|
| 10
| July 26 August 15
|
D102.2
|
D103.1
| [http://partsregistry.org/Part:BBa_J23101 J23101]
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| SpeI
| PstI
| BV
| 2100
| 883
|
|
| 27
| July 26
|
D103.2
| 35
|
D104.1
| [http://partsregistry.org/Part:BBa_J23109 J23109]
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| SpeI
| PstI
| BV
| 2100
| 883
|
|
| 20
| July 26
|
D104.2
|
D105.1
| [http://partsregistry.org/Part:BBa_R0079 R0079]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| 2222
| 14
|
|
| 5
| July 26
|
D105.2
|
D106.1
| [http://partsregistry.org/Part:BBa_R0040 R0040]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| 2119
| 14
|
|
| 8
| July 26
|
D106.2
|
D107
| [http://partsregistry.org/Part:BBa_S03154 S03154]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| 2750
| 14
|
| August 23
| 5 12
| July 26 July 23
|
D108.1
| [http://partsregistry.org/Part:BBa_S03154 S03154]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 707
|
|
| 5
| July 29
|
D108.2
|
D109
| [http://partsregistry.org/Part:BBa_S03154 S03154]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| 2056
| 708
|
| July 26
| 10
| July 26
|
D110
| [http://partsregistry.org/Part:BBa_S03879 S03879]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| 2768
| 14
|
| August 23
| 20 8 19
| July 26 August 23 August 28
|
D111.1
| [http://partsregistry.org/Part:BBa_S03879 S03879]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 725
|
|
| 20
| July 26
|
D111.2
|
D112
| [http://partsregistry.org/Part:BBa_S03879 S03879]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| 2079
| 703
|
|
| 8
| September 5
|
D113
| [http://partsregistry.org/Part:BBa_C0079 C0079]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| EcoRI
| SpeI
| FI
| 4402
| 779
|
|
| 20
| July 26
|
D114
| [http://partsregistry.org/Part:BBa_C0079 C0079]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| XbaI
| PstI
| BI
| 4003
| 778
|
|
| 20
| July 26
|
D115.1
| [http://partsregistry.org/Part:BBa_C0179 C0179]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| 4402
| 746
|
|
| -
| July 29
|
D115.2
|
D116.1
| [http://partsregistry.org/Part:BBa_C0179 C0179]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 4403
| 745
|
|
| 20
| July 29
|
D116.1
|
D117
| [http://partsregistry.org/Part:BBa_E0030 E0030]
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| EcoRI
| SpeI
| FI
| 3166
| 746
|
|
| 5
| July 29
|
D118
| [http://partsregistry.org/Part:BBa_E0030 E0030]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| XbaI
| PstI
| BI
| 3167
| 745
|
|
| 20
| July 26
|
D119
| [http://partsregistry.org/Part:BBa_E0040 E0040]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| 2056
| 743
|
|
| 10
| July 26
|
D120
| [http://partsregistry.org/Part:BBa_E0040 E0040]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 742
|
|
| 10
| July 26
|
D121
| [http://partsregistry.org/Part:BBa_E1010 E1010]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| EcoRI
| SpeI
| FI
| 4402
| 704
|
|
| 10
| July 26
|
D122
| [http://partsregistry.org/Part:BBa_E1010 E1010]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 4403
| 703
|
|
| 10
| July 26
|
D123.1
| [http://partsregistry.org/Part:BBa_J23100 J23100]
| [http://partsregistry.org/Part:J61002 J61002]
| SpeI
| PstI
| BV
| 2100
| 883
|
|
| 26
| July 26
|
D123.2
| 23
|
D124.1
| [http://partsregistry.org/Part:BBa_J23107 J23107]
| [http://partsregistry.org/Part:J61002 J61002]
| SpeI
| PstI
| BV
| 2100
| 883
|
|
| 15
| July 26
|
D124.2
|
D125.1
| [http://partsregistry.org/Part:BBa_B0015 B0015]
| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| EcoRI
| XbaI
| FV
| 3303
| 15
|
|
| 26 10
| July 26 August 23 August 28
|
D125.2
| 24 3687
|
D126.1
| [http://partsregistry.org/Part:BBa_I0500 I0500]
| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| SpeI
| PstI
| BV
| 5621
| 14
|
|
| 10
| July 26
|
D126.2
|
D127.1
| [http://partsregistry.org/Part:BBa_B0030 B0030]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 37
|
|
| 10
| July 26
|
D127.2
|
D128.1
| [http://partsregistry.org/Part:BBa_B0030 B0030]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
| 2079
| 15
|
|
| 10
| July 29
|
D128.1
|
D129.1
| [http://partsregistry.org/Part:BBa_B0030 B0030]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| 2080
| 14
|
|
| 10
| July 29
|
D129.2
|
D130.1
| [http://partsregistry.org/Part:BBa_E0422 E0422]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 939
|
|
| 10
| July 26
|
D130.2
|
D130.3
|
D131.1
| [http://partsregistry.org/Part:BBa_E0840 E0840]
| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 900
|
|
| 8
| July 26
|
D131.2
|
D131.3
|
D132
| pFlgA
| -
| EcoRI
| SpeI
| FI
| -
| 250
|
|
| 5
| August 6
|
D133
| pFlgB
| -
| EcoRI
| SpeI
| FI
| -
| 250
|
|
| 5
| August 6
|
D134
| pFlhB
| -
| EcoRI
| SpeI
| FI
| -
| 249
|
|
| 5
| August 6
|
D135
| pFlhDC
| -
| EcoRI
| SpeI
| FI
| -
|
| problem
|
|
| August 6
|
D136
| -
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| EcoRI
| SpeI
| FV
| 2925
| -
|
|
|
| August 6 August 16
|
D137
| pSB3K3
| [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]
| EcoRI
| SpeI
| FV
| 2727
| -
|
|
|
| August 6
|
D138
| E0240
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| 899
| -
|
|
|
| August 6
|
D139
| flhDC
| -
| EcoRI
| PstI
| FI
| -
| 972
|
|
|
| August 6
|
D140
| E040 rbs+
| -
| PstI
| PstI
| I
| -
|
|
|
|
| August 12
|
D141
| flhD RBS -
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 12
|
D142
| flhC RBS -
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 12
|
D143
| flhDC + promoter
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 12
|
D144
| pSB3K3
| [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 12
|
D145
| pSB1A2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 12 August 15
|
D146
| MP148
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| 2057
| 1609
| rbs TetR - GFP tripart D110 (BV) - D131 (BI)
|
|
| August 15
|
D147
| MP153
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| 2056
| 799
| Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)
|
|
| August 15
|
D149
| pfliL
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 14 August 16
|
D150
| pfliL
| -
| XbaI
| PstI
| BI
| -
|
|
|
|
| August 14 August 16
|
D152
| pSB3K3
| [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]
| XbaI
| PstI
| BI
| -
|
|
|
|
| August 14
|
D153
| PCR 138 - gène flhDC
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 15
|
D154
| PCR 140 - gène flhDC
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 15
|
D155
| PCR 139 - pflhDC (URI)
| -
| EcoRI
| SpeI
| FI
| -
|
|
|
|
| August 15
|
D158
| MP 155.1 - OmpR*
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| -
|
|
|
|
| August 15
|
D159
| MP 156.1 - EnvZ*
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| -
|
|
|
|
| August 15
|
D161
| MP 104 - pTet
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
| -
|
|
|
| 39
| August 16
|
D162
| MP 114 - TetR
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
| -
|
|
|
| 11
| August 16
|
D163
| MP 143 - gfp generator
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
| -
|
|
| August 23
| 14 11
| August 16 August 23
|
D164
| MP 101 - pJ23101
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| SpeI
| PstI
| BV
| -
|
|
|
| 3
| August 16
|
D165
| MP162.2
| [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]
| XbaI
| PstI
| BI
| 2057
| 798
| rbs-lasR - double terminator D147 (FI) - D125 (FV)
|
| 27
| August 18
|
D166
| MP 165.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
| -
| 2942
| RBS (B0034)+ YFP+ LVA- term B0015
|
| 59
| August 26 September 3
|
D179
| MP3.4 or MP118
| [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]
| SpeI
| PstI
| BV
|
|
| B0015 (double terminator B0010-B0012)
|
|
| August 20
|
D180
| MP101.1
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| SpeI
| PstI
| BV
|
|
| promoter J23101
|
|
| August 20
|
D181
| MP104.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
|
|
| pTet (TetR repressible promoter)
|
|
| August 20
|
D182
| MP114.1
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
|
|
| TetR
|
|
| August 20
|
D183
| MP119.3
| -
| XbaI
| PstI
| BI
|
|
| pBad promoter
|
|
| August 20
|
D184
| MP143.1
| [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3]
| EcoRI
| SpeI
| FI
|
|
| gfp generator
|
|
| August 18
|
D185
| MP163.1
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
|
|
| B0032 RBS
|
|
| August 18
|
D187
| MP168.1
| [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3]
| EcoRI
| XbaI
| FV
|
|
| RBS (B0034)+ mRFP LVA+ term B0015
|
| 43
| September 3
|
D188
| PCR 138
| -
| XbaI
| PstI
| Insert
| -
| 767
| OmpR*
|
|
| August 26
|
D189
| MP 101.2
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| XbaI
| PstI
| Vector
| -
| 2073
| pSB1A2
|
|
| August 26
|
D191
| MP114.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
|
| 2724
| tetR
|
| 22
| August 29
|
D192
| MP144.2
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| EcoRI
| XbaI
| FV
|
| 2267
| FlgA
|
| 26
| August 29
|
D193
| MP171
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
|
| 961
| gfp generator(E0240) - pTet
|
| 9,5
| August 29
|
D194
| MP172.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| SpeI
| FI
|
| 704
| RBS(B0032) - tetR
|
| 13
| August 29
|
D195
| MP172.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
|
| 703
| RBS(B0032) - tetR
|
| 11
| August 29
|
D196
| MP104.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| SpeI
| PstI
| BV
|
| 2119
| pTet
|
| 45
| September 3
|
D197
| MP157.2
| [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]
| XbaI
| PstI
| BI
|
| 898
| gfp generator(E0240)
|
| 12
| September 3
|
D198
| MP164.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
|
| 2981
| RBS (B0034)+ ECFP+ LVA+ term B0015
|
| 36
| September 3
|
D199
| MP167.1
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
|
| 2942
| RBS (B0034)+ ECFP LVA- term (B0015)
|
| 61
| September 3
|
D200
| MP171.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| XbaI
| PstI
| BI
|
| 960
| gfp generator(E0240) - pTet
|
| 43
| September 3
|
D201
| MP114.2
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]
| EcoRI
| XbaI
| FV
|
| 2724
| tetR
|
| 13
| August 29
|
D202
| MP101.1
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| EcoRI
| nothing
|
|
| 2984
|
|
| 25
| September 8
|
D203
| MP101.2
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| BspHI
| nothing
|
|
| 1870 1008 105
|
|
|
| September 11
|
D204
| MP144
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| SpeI
| PstI
|
|
|
|
|
|
| September 22
|
D205
| MP101
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| XbaI
| PstI
|
|
|
|
|
|
| September 22
|
D206
| MP179
|
| XbaI
| PstI
|
|
|
|
|
|
| September 22
|
D207
| MP182.3
|
| XbaI
| PstI
|
|
|
|
|
|
| September
|
D208
| MP175
|
| EcoRI
| SpeI
|
|
|
|
|
|
| September
|
D209
| MP144
| [http://partsregistry.org/Part:BBa_J61002 J61002]
| EcoRI
| SpeI
|
|
|
|
|
|
| September
|
D210
| MP178
|
| EcoRI
| SpeI
|
|
|
|
|
|
| September
|