Team:Bologna/Notebook
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!align="center"|[[Team:Bologna|HOME]] | !align="center"|[[Team:Bologna|HOME]] | ||
- | !align="center"|[[Team:Bologna/Project| | + | !align="center"|[[Team:Bologna/Project|PROJECT]] |
- | !align="center"|[[Team:Bologna/Team| | + | !align="center"|[[Team:Bologna/Team|TEAM]] |
- | !align="center"|[[Team:Bologna/Parts| | + | !align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]] |
!align="center"|[[Team:Bologna/Modeling|MODELING]] | !align="center"|[[Team:Bologna/Modeling|MODELING]] | ||
- | !align="center"|[[Team:Bologna/Notebook| | + | !align="center"|[[Team:Bologna/AA|WETLAB]] |
- | !align="center"|[[Team:Bologna/Biosafety|BIOSAFETY]] | + | !align="center"|[[Team:Bologna/Notebook|LAB-BOOK]] |
+ | !align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]] | ||
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Revision as of 14:41, 22 October 2008
HOME | PROJECT | TEAM | SUBMITTED PARTS | MODELING | WETLAB | LAB-BOOK | BIOSAFETY AND PROTOCOLS |
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Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Contents |
Protocols
Protocol | Biosafety level |
Plates preparation | |
Amplification | |
Transformation | |
Inoculation | |
Miniprep | |
Digestion reaction | |
Gel preparation | |
Electrophoretic run | |
Gel extraction | |
Ligation reaction | |
Chemiocompetent cells | |
Mediums and buffers | |
Antibiotics stocks preparation | |
IPTG stocks preparation | |
Fluorescence test | |
M9 supplemented media | |
Week 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
Week 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
Week 3: from 08/04/08 to 08/10/08