Team:Bologna/Notebook

From 2008.igem.org

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!align="center"|[[Team:Bologna|HOME]]
!align="center"|[[Team:Bologna|HOME]]
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!align="center"|[[Team:Bologna/Project|THE PROJECT]]
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!align="center"|[[Team:Bologna/Project|PROJECT]]
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!align="center"|[[Team:Bologna/Team|THE TEAM]]
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!align="center"|[[Team:Bologna/Team|TEAM]]
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!align="center"|[[Team:Bologna/Parts|PARTS SUBMITTED TO THE REGISTRY]]
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!align="center"|[[Team:Bologna/Parts|SUBMITTED PARTS]]
!align="center"|[[Team:Bologna/Modeling|MODELING]]
!align="center"|[[Team:Bologna/Modeling|MODELING]]
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!align="center"|[[Team:Bologna/Notebook|NOTEBOOK]]
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!align="center"|[[Team:Bologna/AA|WETLAB]]
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!align="center"|[[Team:Bologna/Biosafety|BIOSAFETY]]
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!align="center"|[[Team:Bologna/Notebook|LAB-BOOK]]
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!align="center"|[[Team:Bologna/Biosafety|BIOSAFETY AND PROTOCOLS]]
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Revision as of 14:41, 22 October 2008

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HOME PROJECT TEAM SUBMITTED PARTS MODELING WETLAB LAB-BOOK BIOSAFETY AND PROTOCOLS




Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

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Contents

Protocols

For more information, see Biosafety page
Protocol Biosafety level
Plates preparation
1
Amplification
1
Transformation
1
Inoculation
1
Miniprep
1
Digestion reaction
1
Gel preparation
2
Electrophoretic run
2
Gel extraction
2
Ligation reaction
1
Chemiocompetent cells
1
Mediums and buffers
1
Antibiotics stocks preparation
1
IPTG stocks preparation
1
Fluorescence test
1
M9 supplemented media
1



Week 1: from 07/21/08 to 07/27/08

  1. Enzymatic digestion of: C0051 E\S, LACI* X\P.
  2. Gel preparation , electrophoretic run.
  3. Amplification of: TETR , PUV, PRECA.


Week 2: from 07/28/08 to 08/03/08

  1. Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
  2. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240


Week 3: from 08/04/08 to 08/10/08