Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
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= Genetic toggle switch to implement rewritable function= | = Genetic toggle switch to implement rewritable function= | ||
In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). | In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). | ||
- | We think two | + | We have to think two types of model - genetic circuit model and mathematical model - so as to construct PIGTS. |
== Genetic circuit model == | == Genetic circuit model == |
Revision as of 08:39, 24 October 2008
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Genetic toggle switch to implement rewritable function
In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). We have to think two types of model - genetic circuit model and mathematical model - so as to construct PIGTS.
Genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure(PL is weakly induced)
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
Design of pressure inducible genetic toggle switch(in short PIGTS) We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function.
- Converge to high CI concentration state under 30MPa pressure (writable)
- Bistable under ordinary pressure (erasable)