Team:Warsaw/Calendar-Main/11 July 2008

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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
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  primers on colonies from plates with transformations OmpA_alpha (45 s of elongation step). </li>
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  primers on colonies from plates with transformations OmpA_alpha (annealin temperature - 55 deg;C,45 s of elongation step). </li>
<li> Gel electrophoresis.</li>
<li> Gel electrophoresis.</li>
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li>
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li>

Revision as of 16:08, 24 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

  1. Colony PCR with OmpaL_N and OmpaP_link primers on colonies from plates with transformations OmpA_alpha (annealin temperature - 55 deg;C,45 s of elongation step).
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of construct pKS with A protein

Michał L., Ewa, Marcin

  1. Colony PCR on colonies from plates with transformations pKS-A.
    Primers used: AL+SacI and AP+NotI
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with ampicillin.

Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA

Piotr, Antoni

Isolation of plasmids from cultures inoculated on previous day.