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Team:Warsaw/Calendar-Main/11 July 2008
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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
| - | primers on colonies from plates with transformations OmpA_alpha (45 s of elongation step). </li> | + | primers on colonies from plates with transformations OmpA_alpha (annealin temperature - 55 deg;C,45 s of elongation step). </li> |
<li> Gel electrophoresis.</li> | <li> Gel electrophoresis.</li> | ||
<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | <li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | ||
Revision as of 16:08, 24 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, AntoniIsolation of plasmids from cultures inoculated on previous day. |
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