Team:ETH Zurich
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Welcome to the ETH Zurich iGEM Wiki, the web site is under heavy reconstruction so please be patient if most of the links are not available.<br> | Welcome to the ETH Zurich iGEM Wiki, the web site is under heavy reconstruction so please be patient if most of the links are not available.<br> | ||
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- | + | == Project Abstract == | |
''''''Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome''''''<br> | ''''''Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome''''''<br> | ||
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+ | == Site Map == | ||
+ | {| border="1" | ||
+ | |+ Site Map of the ETHZ Side | ||
+ | ! Home !! Team !! Project !! Wetlab !! Modeling !! Tools | ||
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+ | | [https://2008.igem.org/Team:ETH_Zurich Overview] | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Team/Overview Overview] | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Project/Overview Overview] | ||
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+ | | [https://2008.igem.org/Team:ETH_Zurich ETH Zuerich] | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Team/Members Members] | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Project/Motivation Motivation] | ||
+ | |- | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Sponsors Sponsors] | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Team/Pictures Pictures] | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Project/Background Background] | ||
+ | |- | ||
+ | | [https://2008.igem.org/Team:ETH_Zurich/Project/Applications Applications] | ||
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+ | | [https://2008.igem.org/Team:ETH_Zurich/Project/Conclusions Conclusions] | ||
+ | |} | ||
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Revision as of 07:15, 25 October 2008
Project Abstract'Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome' "This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome.
Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering.
We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is base on an iterative cycle of genome reduction and strain selection.
We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based selective condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection." Site Map
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