Team:Warsaw/Calendar-Main/12 July 2008
From 2008.igem.org
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> | ||
- | <li>One of positive plasmids transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB+amp, overnight, for further isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b | + | <li>One of positive plasmids transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB+amp, overnight, for further isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z+alpha</a> vector.</li> |
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Revision as of 18:31, 25 October 2008
Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, Antoni
1. Marker 2-4. digested plasmids which had a colony PCR product |