Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
From 2008.igem.org
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* '''Plac''' is induced under 30MPa pressure | * '''Plac''' is induced under 30MPa pressure | ||
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* Express CI and GFP | * Express CI and GFP | ||
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- | [[Image:Desirable_condition.png|frame|left|fig5-4:Desirable condition. left:under atmospheric pressure (nullclines of fig:5-2), right:under 30MPa pressure (nullclines of fig:5-3).] | + | [[Image:Desirable_condition.png|frame|left|fig5-4:Desirable condition. left:under atmospheric pressure (nullclines of fig:5-2), right:under 30MPa pressure (nullclines of fig:5-3).]] |
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- | + | It is known that Plac activity under 30MPa pressure is '''94.0''' times stronger than Plac activity under atmospheric pressure, so, '''β<sub>30P<sub>lac</sub></sub>''' = 94.0. | |
we had to identify the domain of appropriate parameters. | we had to identify the domain of appropriate parameters. | ||
Revision as of 20:30, 25 October 2008
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Genetic toggle switch to implement rewritable function
In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). We have to think two types of model - genetic circuit model and mathematical model - so as to construct PIGTS.
Genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP(inserted destruction tags) ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
We used two differential equation models and we call them 'atmospheric pressure model' and '30MPa pressure model'. In order to implement rewritable function, we decided to use the difference of nullclines of each system so as to satisfy the following condition
- Converge to high CI concentration state under 30MPa pressure (writable)
- Bistable under atmospheric pressure (erasable)
It is known that Plac activity under 30MPa pressure is 94.0 times stronger than Plac activity under atmospheric pressure, so, β30Plac = 94.0. we had to identify the domain of appropriate parameters.
Identify the domain of appropriate parameters
Two systems have six parameters. We have already known β30Plac = 94.0 and nPL = 3.0 from earlier studies, and we identified nPlac = 2.15 by our activity determination. And we can adjust promoter - RBS strength, so we had just consider the ratio of αPlac and αPL. Therefore, we can fix αPlac.
We identified β30PL = 1.42 by our experiment under 30MPa pressure. Therefore, we can implement rewritable function if we choose PL - RBS strength range from 2.4 to 7.4.