Team:Warsaw/Calendar-Main/11 July 2008
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- | < | + | <h3>Preparation of construct <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS with A protein</a></h3> |
- | + | <h4>Michał L., Ewa, Marcin</h4> | |
- | + | <p><ol> | |
- | + | <li> Colony PCR on colonies from plates with transformations <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII+A>pKS-A</a>. <br> | |
- | + | Primers used: | |
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+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> | ||
+ | </li><li> Gel electrophoresis.</li> | ||
+ | <li> Confirmed transformant colonies inoculated to liquid LB with ampicillin.</li></ol></p> | ||
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+ | <h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b-OmpA-alpha</a> in place of OmpA</h3> | ||
+ | <h4>Piotr, Antoni</h4> | ||
+ | <p><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day.</p> | ||
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+ | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega #2</h3> | ||
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+ | <h4>Michał K.</h4> | ||
+ | <p><ol> | ||
+ | <li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
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+ | primers on colonies from plates with transformations OmpA_alpha (annealing temperature - 55°C,45 s of elongation step). </li> | ||
+ | <li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_July_2008#fig1">Fig. 1.</a>).</li> | ||
+ | <li> Confirmed transformant colonies inoculated to liquid LB with kanamycin.</li> | ||
+ | </ol></p> | ||
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+ | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/7e/11_th_july.jpg" width=300 /></a><var><b>Fig. 1. </b>Colony PCR using: OmpaL_N and OmpaP_link primers<b></b><br> | ||
+ | Upper<br> | ||
+ | 1. Marker<br> | ||
+ | 2-13. colony PCR products carrying probable OmpA-alpha<br> | ||
+ | Lower<br> | ||
+ | 1. Marker<br> | ||
+ | 2-13. colony PCR products carrying probable OmpA-alpha<br></var> | ||
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+ | </html> | ||
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Latest revision as of 17:44, 26 October 2008
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpAPiotr, AntoniIsolation of plasmids from cultures inoculated on previous day. Preparation of constructs: OmpA_alpha and OmpA_omega #2Michał K.
Upper 1. Marker 2-13. colony PCR products carrying probable OmpA-alpha Lower 1. Marker 2-13. colony PCR products carrying probable OmpA-alpha |