Team:Hawaii/Notebook/2008-10-26

From 2008.igem.org

(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> [[Image:102608plasmid.png|right|thumb|EtBr stained 0.8% agarose gel ran at 95V...)
(Plasmid prep)
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:* Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
:* Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
:* Ran on gel to verify plasmid
:* Ran on gel to verify plasmid
-
 
+
::* ~2ng/&mu;l
 +
::* The prep kit yields really clean preps in ~1 hour. :D!
= Discussion =
= Discussion =

Revision as of 21:55, 26 October 2008

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Things we did today

Wetlab work

Plasmid prep

Grace
EtBr stained 0.8% agarose gel ran at 95V for 1 hour. Ten microliters of plasmid were loaded into each well.
  • Used Qiaprep Spin Miniprep Kit to plasmid prep BB1 (10/22 and 10/25 inoculations)
  • Ran on gel to verify plasmid
  • ~2ng/μl
  • The prep kit yields really clean preps in ~1 hour. :D!

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]