Team:Bologna/Parts
From 2008.igem.org
(Difference between revisions)
(→Composites) |
(→Composites) |
||
Line 100: | Line 100: | ||
| [http://partsregistry.org/Part:BBa_K079036 K079036] | | [http://partsregistry.org/Part:BBa_K079036 K079036] | ||
| Tet O operator library member | | Tet O operator library member | ||
- | | | + | | GENEART pMA |
| 15 | | 15 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 108: | Line 108: | ||
| [http://partsregistry.org/Part:BBa_K079037 K079037] | | [http://partsregistry.org/Part:BBa_K079037 K079037] | ||
| TetO-4C - operator library member | | TetO-4C - operator library member | ||
- | | | + | | GENEART pMA |
| 15 | | 15 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 116: | Line 116: | ||
| [http://partsregistry.org/Part:BBa_K079038 K079038] | | [http://partsregistry.org/Part:BBa_K079038 K079038] | ||
| TetO-wt/4C5G - operator library member | | TetO-wt/4C5G - operator library member | ||
- | | | + | | GENEART pMA |
| 15 | | 15 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 124: | Line 124: | ||
| [http://partsregistry.org/Part:BBa_K079039 K079039] | | [http://partsregistry.org/Part:BBa_K079039 K079039] | ||
| LexA 1 - operator library member | | LexA 1 - operator library member | ||
- | | | + | | GENEART pMA |
| 16 | | 16 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 132: | Line 132: | ||
| [http://partsregistry.org/Part:BBa_K079040 K079040] | | [http://partsregistry.org/Part:BBa_K079040 K079040] | ||
| LexA 2 - opeartor library member | | LexA 2 - opeartor library member | ||
- | | | + | | GENEART pMA |
| 16 | | 16 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 140: | Line 140: | ||
| [http://partsregistry.org/Part:BBa_K079041 K079041] | | [http://partsregistry.org/Part:BBa_K079041 K079041] | ||
| Lambda OR1 - operator library member | | Lambda OR1 - operator library member | ||
- | | | + | | GENEART pMA |
| 17 | | 17 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 148: | Line 148: | ||
| [http://partsregistry.org/Part:BBa_K079042 K079042] | | [http://partsregistry.org/Part:BBa_K079042 K079042] | ||
| Lambda OR2 - operator library member | | Lambda OR2 - operator library member | ||
- | | | + | | GENEART pMA |
| 17 | | 17 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 156: | Line 156: | ||
| [http://partsregistry.org/Part:BBa_K079043 K079043] | | [http://partsregistry.org/Part:BBa_K079043 K079043] | ||
| Lambda OR3 - operator library member | | Lambda OR3 - operator library member | ||
- | | | + | | GENEART pMA |
| 17 | | 17 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 172: | Line 172: | ||
| [http://partsregistry.org/Part:BBa_K079046 K079046] | | [http://partsregistry.org/Part:BBa_K079046 K079046] | ||
| Tet operator library | | Tet operator library | ||
- | | | + | | GENEART pMA |
| 61 | | 61 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 180: | Line 180: | ||
| [http://partsregistry.org/Part:BBa_K079047 K079047] | | [http://partsregistry.org/Part:BBa_K079047 K079047] | ||
| Lambda operator library | | Lambda operator library | ||
- | | | + | | GENEART pMA |
| 67 | | 67 | ||
| <center>Yes</center> | | <center>Yes</center> | ||
Line 188: | Line 188: | ||
| [http://partsregistry.org/Part:BBa_K079048 K079048] | | [http://partsregistry.org/Part:BBa_K079048 K079048] | ||
| LexA operator library | | LexA operator library | ||
- | | | + | | GENEART pMA |
| 40 | | 40 | ||
| <center>Yes</center> | | <center>Yes</center> |
Revision as of 18:42, 27 October 2008
HOME | TEAM | PROJECT | MODELING | WET-LAB | SOFTWARE | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
---|
Composites
The table below shows all the composites that we have obtained to build up our final device. For each part you can find:
-in the second column the link to the Registry;
-in the third column the components for each part;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
N° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| [http://partsregistry.org/Part:BBa_K079015 K0790015] | LacY transporter protein from E. coli | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] | 1254 | | |
| [http://partsregistry.org/Part:BBa_K079020 K079020] | LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3 (HC)] | 2165 | | |
| [http://partsregistry.org/Part:BBa_K079029 K079029] | GFP expression controlled by LacI repressor under the BBa_J23114 promoter | 2501 | | | |
| [http://partsregistry.org/Part:BBa_K079017 K079017] | Lac symmetric - operator library member | GENEART pGA18 | 20 | | |
| [http://partsregistry.org/Part:BBa_K079018 K079018] | Lac 1 - operator library member | GENEART pGA18 | 21 | | |
| [http://partsregistry.org/Part:BBa_K079019 K079019] | Lac 2 - operator library member | GENEART pGA18 | 21 | | |
| [http://partsregistry.org/Part:BBa_K079036 K079036] | Tet O operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079037 K079037] | TetO-4C - operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079038 K079038] | TetO-wt/4C5G - operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079039 K079039] | LexA 1 - operator library member | GENEART pMA | 16 | | |
| [http://partsregistry.org/Part:BBa_K079040 K079040] | LexA 2 - opeartor library member | GENEART pMA | 16 | | |
| [http://partsregistry.org/Part:BBa_K079041 K079041] | Lambda OR1 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079042 K079042] | Lambda OR2 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079043 K079043] | Lambda OR3 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079045 K079045] | Lac operator library | GENEART pGA18 | 78 | | |
| [http://partsregistry.org/Part:BBa_K079046 K079046] | Tet operator library | GENEART pMA | 61 | | |
| [http://partsregistry.org/Part:BBa_K079047 K079047] | Lambda operator library | GENEART pMA | 67 | | |
| [http://partsregistry.org/Part:BBa_K079048 K079048] | LexA operator library | GENEART pMA | 40 | | |
| [http://partsregistry.org/Part:BBa_K079049 K079049] | GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 981 | | |
| [http://partsregistry.org/Part:BBa_K079050 K079050] | GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 981 | | |
| [http://partsregistry.org/Part:BBa_K079023 K079023] | LacI repressor under the control of the J23118 constitutive promoter and Lac 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 1243 | | |
| [http://partsregistry.org/Part:BBa_K079023 K079024] | LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 1243 | | |
| [http://partsregistry.org/Part:BBa_K079026 K079026] | GFP reporter protein regulated by J23118 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2116 | | |
| [http://partsregistry.org/Part:BBa_K079030 K079030] | GFP reporter protein controlled by the BBa_23105 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |
| [http://partsregistry.org/Part:BBa_K079031 K079031] | GFP reporter protein under the control of the BBa_J23118 promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |
| [http://partsregistry.org/Part:BBa_K079032 K079032] | GFP reporter protein under the control of the BBa_J23100 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |