Team:Michigan/Project
From 2008.igem.org
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== <font color=dodgerblue size=4><B>Our Project: The Sequestillator</B></font> == | == <font color=dodgerblue size=4><B>Our Project: The Sequestillator</B></font> == | ||
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<div align=center>[[Image:Combined topology.png]]</div> | <div align=center>[[Image:Combined topology.png]]</div> | ||
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+ | We can subdivide our clock into three parts: the tetR system, the activator module, and the repressor module. The activator module consists of the tet promoter driving the NifA gene. The NifA protein binds to the nifHp promoter of the repressor module, activating transcription of the NifL gene. Once NifL dimerizes, it can bind to the NifA hexamer, hence preventing NifA from binding to NifHp. This sequestration effect provides the clock's negative feedback loop that is essential for oscillations. | ||
+ | The tetR system regulates the levels of nifA. At its resting state, tetR completely represses the production of NifA. However, addition of anhydrotetracycline prevents the Tet protein from binding to tet promoter, allowing for a "constitutive" production of NifA as long as the levels of anhydrotetracycline are kept constant. This module allows us to tune the levels of NifA by adjusting the amount of anhydrotetracycline we add. | ||
== <font color=dodgerblue size=4><B>Landing Pads</B></font> == | == <font color=dodgerblue size=4><B>Landing Pads</B></font> == |
Revision as of 21:36, 27 October 2008
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Sequestilator ModelingIf you like the way this looks, you could put a summary of what you modeled here and then we can have a separate page for modeling, which might be a good idea. |
Sequestilator FabricationIf you like the way this looks, you could put a summary of what you built here and then we can have a separate page for fabrication, which might be a good idea. |
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