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Team:Princeton/Experiments
From 2008.igem.org
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===Bio-brick Creation=== | ===Bio-brick Creation=== | ||
'''Lab Protocols''' | '''Lab Protocols''' | ||
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1. Primer Design | 1. Primer Design | ||
2. PCR | 2. PCR | ||
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13. Extract DNA (Maxiprep or Midiprep) | 13. Extract DNA (Maxiprep or Midiprep) | ||
14. Sequence | 14. Sequence | ||
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Cell Culture | Cell Culture | ||
Revision as of 08:25, 28 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
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| Parts Submitted to the Registry | Modeling | The Team | Gallery |
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Bio-brick Creation
Lab Protocols
1. Primer Design 2. PCR 3. Run Gel 4. Gel Extraction 5. Digestion 6. CIP Treatment 7. PCR Purification 8. Ligation 9. Transformation and Plating 10. Extract DNA (Miniprep) 11. Restriction Map/ Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence
Cell Culture
