Team:Princeton/Experiments

From 2008.igem.org

(Difference between revisions)
(→Bio-brick Creation)
(→Bio-brick Creation)
Line 25: Line 25:
10. [[Extract DNA (Miniprep)]]
10. [[Extract DNA (Miniprep)]]
-
11. [[Restriction Map/ Digest]]
+
11. [[Restriction Map/ Restriction Digest]]
12. [[Re-transform with selected plasmid]]
12. [[Re-transform with selected plasmid]]

Revision as of 17:34, 28 October 2008

PrincetonLogo.gif

PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. PCR SOEing

4. Run Gel/ Gel extraction

5. Digestion

6. CIP Treatment

7. PCR Purification

8. Ligation

9. Transformation and Plating

10. Extract DNA (Miniprep)

11. Restriction Map/ Restriction Digest

12. Re-transform with selected plasmid

13. Extract DNA (Maxiprep or Midiprep)

14. Sequence

Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

Retrieved from "http://2008.igem.org/Team:Princeton/Experiments"