Team:Warsaw/Calendar-Main/2 October 2008
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<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=http://partsregistry.org/Part:BBa_K103018>BBa_K103018</a> fragment with EcoRI and BcuI (BamHI buffer). </li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=http://partsregistry.org/Part:BBa_K103018>BBa_K103018</a> fragment with EcoRI and BcuI (BamHI buffer). </li> | ||
- | <li> Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 1200 bp. <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008#fig3">Fig. 3 | + | <li> Gel electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 1200 bp. <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/2_October_2008#fig3">Fig. 3</a>.</li> |
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Revision as of 20:33, 28 October 2008
Preparation of linker_alpha (BBa_K103009)Michał K.Overnight ligation of isolated DNA fragments: pSB2K3 + linker_alpha (BBa_K103009). Preparation of linker_omega (BBa_K103013)Michał K.Overnight ligation of isolated DNA fragments: pSB2K3 + linker_omega (BBa_K103013). Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.Overnight ligation of DNA fragments isolated on 30 September : pACYC177 + OmpA-linker-omega-linker (BBa_K103016). Preparation of OmpA-linker (BBa_K103006)Michał K.
1. Marker 4, 14, 19, 20, 24. Proper bands visible. Preparation of Z(BBa_K103004)Michał K.
1. Marker 3-5. Clones carrying BBa_K103004 Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)Michał K.
1. Marker 2. OmpA_linker_omega_linker under Plac (BBa_K103018)
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