Team:Freiburg/3D-Modeling
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<font face="Arial Rounded MT Bold" style="color:#010369">_3d-Modeling</font></div> | <font face="Arial Rounded MT Bold" style="color:#010369">_3d-Modeling</font></div> | ||
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== DNA-Origami == | == DNA-Origami == | ||
Planning the input pattern on the DNA-Origami surface, we generated various 3D-models of the huge molecule using "Nano-Enineer" (a free program in its beta-version), Pymol and SwissPdbViewer (both freeware). Some of those models are shown here to give you an impression of the molecules we have engineered this year.<br> | Planning the input pattern on the DNA-Origami surface, we generated various 3D-models of the huge molecule using "Nano-Enineer" (a free program in its beta-version), Pymol and SwissPdbViewer (both freeware). Some of those models are shown here to give you an impression of the molecules we have engineered this year.<br> | ||
[[Image:Freiburg2008_Fab_on_Origami_animated.gif|800 px]] | [[Image:Freiburg2008_Fab_on_Origami_animated.gif|800 px]] | ||
- | + | '''Fig.1:'''Some of the oligo nucleotides that shape the single-strand template fused to NIP-molecules and an anti-NIP-Fab-Fragment<br><br><br> | |
[[Image:Freiburg08_DNA-NIP.png|400 px]] [[Image:Freiburg08_DNA-NIP5.png|400 px]]<br> | [[Image:Freiburg08_DNA-NIP.png|400 px]] [[Image:Freiburg08_DNA-NIP5.png|400 px]]<br> | ||
- | + | '''Fig.2:'''DNA-Origami with NIP-molecules and anti-NIP-fragment_______'''Fig.3:'''Oligo-pattern with scale and two fragments (dimerization model)<br><br><br> | |
[[Image:Freiburg08_DNA-NIP3.png|800 px]]<br> | [[Image:Freiburg08_DNA-NIP3.png|800 px]]<br> | ||
- | + | '''Fig.4:'''Oligo-pattern with NIP-molecules and Fab-fragment, binding accessability estimation model<br><br><br> | |
== Fusion Proteins == | == Fusion Proteins == | ||
- | + | The pictures below show models of the fusion proteins our composite parts Bba_K157032 and Bba_K157033 code for(generated with "SwissPdbViewer"):<br> | |
[[Image:Team-Freiburg2008_Lipo_alpha_nCFP.png|400 px]][[Image:Team-Freiburg2008_Lipo_alpha_link_cCFP.png|400 px]] | [[Image:Team-Freiburg2008_Lipo_alpha_nCFP.png|400 px]][[Image:Team-Freiburg2008_Lipo_alpha_link_cCFP.png|400 px]] | ||
+ | '''Fig.5: Red:''' LipocalinFluA, '''Grey:''' "Transmembrane region (BCR, no structural data; symbolized by helix), '''Blue:''' '''left:''' Split-Cerulean (CFP), N-terminal fragment; '''right:''' Split-Cerulean (CFP), C-terminal fragment<br> | ||
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Revision as of 22:02, 28 October 2008