Team:University of Ottawa/7 July 2008

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(New page: __TOC__ ==Today in the lab== '''Dan and Chris''' :'''Miniprep''' ::<li>Performed a miniprep on all the innocluated strains :'''Digestion''' ::<li> Digested miniprep constructs with HindIII...)
 
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__TOC__
__TOC__
==Today in the lab==
==Today in the lab==
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:'''Gel'''
:'''Gel'''
::<li>
::<li>
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'''Matt'''
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:'''Gel'''
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::<li> A gel was run for the digestion on Friday but did not turn out correctly - the correct band sizes did not appear.
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::<li> The same digestion was performed again, this time using a little more PstI enzyme. Gel results still gave incrrect band sizes.
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::<li> Performing an inoculation again with new colonies chosen from plates.

Latest revision as of 23:44, 28 October 2008

Untitled Document

 

 

Contents

Today in the lab

Dan and Chris

Miniprep
  • Performed a miniprep on all the innocluated strains
  • Digestion
  • Digested miniprep constructs with HindIII and Atcre with EcoRI
  • Gel
  • Matt

    Gel
  • A gel was run for the digestion on Friday but did not turn out correctly - the correct band sizes did not appear.
  • The same digestion was performed again, this time using a little more PstI enzyme. Gel results still gave incrrect band sizes.
  • Performing an inoculation again with new colonies chosen from plates.