Team:Bologna/Parts
From 2008.igem.org
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-the link to the Registry in the second column ; | -the link to the Registry in the second column ; | ||
- | - part components, in the third column ; | + | -part components, in the third column ; |
-in the fourth column the plasmid the part has been cloned in; | -in the fourth column the plasmid the part has been cloned in; |
Revision as of 16:50, 29 October 2008
HOME | PROJECT | TEAM | SOFTWARE | MODELING | WET LAB | LAB-BOOK | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
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Composites
The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:
-the link to the Registry in the second column ;
-part components, in the third column ;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
N° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| [http://partsregistry.org/Part:BBa_K079015 K079015] | LacY transporter protein from E. coli (for the improvement of the registry) | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1AK3 (HC)] | 1254 | | |
| [http://partsregistry.org/Part:BBa_K079020 K079020] | LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2165 | | |
| [http://partsregistry.org/Part:BBa_K079029 K079029] | GFP expression controlled by LacI repressor under the BBa_J23114 promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2501 | | |
| [http://partsregistry.org/Part:BBa_K079017 K079017] | Lac symmetric - operator library member | GENEART pGA18 | 20 | | |
| [http://partsregistry.org/Part:BBa_K079018 K079018] | Lac 1 - operator library member | GENEART pGA18 | 21 | | |
| [http://partsregistry.org/Part:BBa_K079019 K079019] | Lac 2 - operator library member | GENEART pGA18 | 21 | | |
| [http://partsregistry.org/Part:BBa_K079036 K079036] | Tet O operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079037 K079037] | TetO-4C - operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079038 K079038] | TetO-wt/4C5G - operator library member | GENEART pMA | 15 | | |
| [http://partsregistry.org/Part:BBa_K079039 K079039] | LexA 1 - operator library member | GENEART pMA | 16 | | |
| [http://partsregistry.org/Part:BBa_K079040 K079040] | LexA 2 - opeartor library member | GENEART pMA | 16 | | |
| [http://partsregistry.org/Part:BBa_K079041 K079041] | Lambda OR1 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079042 K079042] | Lambda OR2 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079043 K079043] | Lambda OR3 - operator library member | GENEART pMA | 17 | | |
| [http://partsregistry.org/Part:BBa_K079045 K079045] | Lac operator library | GENEART pGA18 | 78 | | |
| [http://partsregistry.org/Part:BBa_K079046 K079046] | Tet operator library | GENEART pMA | 61 | | |
| [http://partsregistry.org/Part:BBa_K079047 K079047] | Lambda operator library | GENEART pMA | 67 | | |
| [http://partsregistry.org/Part:BBa_K079048 K079048] | LexA operator library | GENEART pMA | 40 | | |
| [http://partsregistry.org/Part:BBa_K079049 K079049] | GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 981 | | |
| [http://partsregistry.org/Part:BBa_K079050 K079050] | GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 981 | | |
| [http://partsregistry.org/Part:BBa_K079023 K079023] | LacI repressor under the control of the J23118 constitutive promoter and Lac 2 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 1243 | | |
| [http://partsregistry.org/Part:BBa_K079023 K079024] | LacI repressor under the control of the J23118 constitutive promoter and Lac 1 operator | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 1243 | | |
| [http://partsregistry.org/Part:BBa_K079026 K079026] | GFP reporter protein regulated by J23118 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 2116 | | |
| [http://partsregistry.org/Part:BBa_K079030 K079030] | GFP reporter protein controlled by the BBa_23105 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |
| [http://partsregistry.org/Part:BBa_K079031 K079031] | GFP reporter protein under the control of the BBa_J23118 promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |
| [http://partsregistry.org/Part:BBa_K079032 K079032] | GFP reporter protein under the control of the BBa_J23100 constitutive promoter | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | 957 | | |