Team:University of Lethbridge/Modeling

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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!align="center"|[[Image:UofLteamlogo.jpg|150px]]
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[{{SERVER}}{{localurl:Team:University_of_Lethbridge|Home}} https://static.igem.org/mediawiki/2008/6/65/UofLhomebutton.jpg]
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[{{SERVER}}{{localurl:Team:University_of_Lethbridge/Modeling}} https://static.igem.org/mediawiki/2008/a/af/UofLmodelingbutton.jpg]
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|align="center"|[[Team:University_of_Lethbridge | Team Example 2]]
 
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<!--- The Mission, Experiments --->
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==<span style="background-color:#000000; color:white">Our Project</span>==
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For our project, we decided to create a synthetic bacterium which could move towards and degrade an environmental pollutant. To direct the movement of our modified "Bacuum Cleaner," we reprogrammed the chemotaxis pathway by using a toxin-responsive riboswitch to control expression of the motility protein CheZ. CheZ catalyzes the dephosphorylation of the motility protein CheY, which promotes directed movement (chemotaxis) over the "tumbling" state of the bacterium.
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We used the modeling program [http://www.pdn.cam.ac.uk/groups/comp-cell/BCT.html <font color=yellow>BCT</font>] (Bray et al, 1993, 2007; Bray and Bourret, 1995) to verify that CheZ expression levels can be manipulated to control bacterial movement.  The program [http://www.pdn.cam.ac.uk/groups/comp-cell/BCT.html <font color=yellow>BCT</font>] simulates the chemotaxis signalling pathway in ''Escherichia coli'' and is extensively supported by [http://www.pdn.cam.ac.uk/groups/comp-cell/Mutants.html <font color=yellow>experimental data</font>]. We used the program to demonstrate that controlling CheZ expression will control bacterial motility and to determine the amount of CheZ necessary to dephosphorylate CheY in high enough levels to produce directed movement. We modeled the E. coli strain RP437 in BCT, which is the same strain our group used as the chassis for our iGEM project.
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Increasing intracellular amounts of CheZ led to increased concentration of CheY in the unphosphorylated state (Figure 1). Motility also increased, from a relative state of 0 (no movement or the tumbling state) to the relative state of 1 (continually moving in a directed manner).
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[[Image:Chez_hj_black.gif|400px]]
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<font color=white> Figure 1
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==<span style="background-color:#000000; color:white">References</span>==
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Bray, D., Bourret, R. B., and Simon, M. I. 1993. Computer simulation of the phosphorylation cascade controlling bacterial chemotaxis. ''Mol. Biol. Cell'' 4, 469-482.
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Bray, D., and Bourret, R. B. 1995. Computer analysis of the binding reactions leading to a transmembrane receptor-linked multiprotein complex involved in bacterial chemotaxis. ''Mol. Biol. Cell'' 6, 1367-1380.
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Bray, D., Levin, M. D., and Lipkow, K. 2007. The chemotactic behavior of computer-based surrogate bacteria. ''Curr. Biol.'' 17, 12-19.
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[{{SERVER}}{{localurl:Team:University_of_Lethbridge/Modeling}} https://static.igem.org/mediawiki/2008/a/af/UofLmodelingbutton.jpg]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:University_of_Lethbridge|Home]]
 
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!align="center"|[[Team:University_of_Lethbridge/Team|The Team]]
 
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!align="center"|[[Team:University_of_Lethbridge/Project|The Project]]
 
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!align="center"|[[Team:University_of_Lethbridge/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:University_of_Lethbridge/Modeling|Modeling]]
 
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!align="center"|[[Team:University_of_Lethbridge/Notebook|Notebook]]
 
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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===Note===
 
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If you choose to include a '''Modeling''' page, please write about your modeling adventures here.  This is not necessary but it may be a nice list to include.
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Latest revision as of 01:00, 30 October 2008

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[http://2008.igem.org/wiki/index.php?title=Team:University_of_Lethbridge&Home UofLhomebutton.jpg]

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UofLteamlogo.jpg

Our Project

For our project, we decided to create a synthetic bacterium which could move towards and degrade an environmental pollutant. To direct the movement of our modified "Bacuum Cleaner," we reprogrammed the chemotaxis pathway by using a toxin-responsive riboswitch to control expression of the motility protein CheZ. CheZ catalyzes the dephosphorylation of the motility protein CheY, which promotes directed movement (chemotaxis) over the "tumbling" state of the bacterium.


We used the modeling program [http://www.pdn.cam.ac.uk/groups/comp-cell/BCT.html BCT] (Bray et al, 1993, 2007; Bray and Bourret, 1995) to verify that CheZ expression levels can be manipulated to control bacterial movement. The program [http://www.pdn.cam.ac.uk/groups/comp-cell/BCT.html BCT] simulates the chemotaxis signalling pathway in Escherichia coli and is extensively supported by [http://www.pdn.cam.ac.uk/groups/comp-cell/Mutants.html experimental data]. We used the program to demonstrate that controlling CheZ expression will control bacterial motility and to determine the amount of CheZ necessary to dephosphorylate CheY in high enough levels to produce directed movement. We modeled the E. coli strain RP437 in BCT, which is the same strain our group used as the chassis for our iGEM project.


Increasing intracellular amounts of CheZ led to increased concentration of CheY in the unphosphorylated state (Figure 1). Motility also increased, from a relative state of 0 (no movement or the tumbling state) to the relative state of 1 (continually moving in a directed manner).

Chez hj black.gif

Figure 1

References

Bray, D., Bourret, R. B., and Simon, M. I. 1993. Computer simulation of the phosphorylation cascade controlling bacterial chemotaxis. Mol. Biol. Cell 4, 469-482.

Bray, D., and Bourret, R. B. 1995. Computer analysis of the binding reactions leading to a transmembrane receptor-linked multiprotein complex involved in bacterial chemotaxis. Mol. Biol. Cell 6, 1367-1380.

Bray, D., Levin, M. D., and Lipkow, K. 2007. The chemotactic behavior of computer-based surrogate bacteria. Curr. Biol. 17, 12-19.

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