Team:BrownTwo/Limiter/altdesign

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*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
*Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)
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In order to modify our limiter to regulate an endogenous gene network, one must study a gene pathway map and identify a single link between the gene of interest and a transcription factor that regulates the expression of that gene.
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A handful of these parts were available in the Registry, but we found it necessary to
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Modularity –
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Stipulation:  If G is a transcription factor that is known to auto-regulate, one will have to alter the modeling to account for this situation.
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modifications to current scheme:
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Possible further modifications to these transcription factors include the use of different regulation domains,
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Involves the consideration of multiple binding sites

Revision as of 01:23, 30 October 2008



Alternative construction and design possiblities

  • up-limiter
  • dual limiter
  • limiter device as a regulatory bridge between two pathways
  • killswitch
  • Smolke’s gal2 knockout scheme for using the GAL1 promoter
  • Finding additional inducible/repressible promoters to use in the system
  • Ribozyme switch inhibition to knockdown leakiness of Repressor#3 (this would need to designed so that the aptamer on the repressor #3 mRNA responds to Repressor #2 protein)

In order to modify our limiter to regulate an endogenous gene network, one must study a gene pathway map and identify a single link between the gene of interest and a transcription factor that regulates the expression of that gene. A handful of these parts were available in the Registry, but we found it necessary to

Modularity –

Stipulation: If G is a transcription factor that is known to auto-regulate, one will have to alter the modeling to account for this situation.

modifications to current scheme:


Possible further modifications to these transcription factors include the use of different regulation domains,

Involves the consideration of multiple binding sites

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