Team:Imperial College/Protocols
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*[[Team:Imperial College/Transformation |Protocol 1]] | *[[Team:Imperial College/Transformation |Protocol 1]] | ||
*[[Team:Imperial College/Transformation_2 |Protocol 2]] | *[[Team:Imperial College/Transformation_2 |Protocol 2]] | ||
- | ====== 2 | + | ====== 2. Transformation Protocol for ''E.coli'' ====== |
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*[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]] | *[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]] | ||
- | ====== | + | ====== 3. PCR ====== |
*[[Team:Imperial College/PCR |PCR]] | *[[Team:Imperial College/PCR |PCR]] | ||
- | ====== | + | ====== 4. Calibration Curves ====== |
*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]] | *[[Team:Imperial College/Calibration_Curves |Calibration Curves ]] | ||
*[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]] | *[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]] | ||
*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]] | *[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]] | ||
- | ====== | + | ====== 5. Promoter-RBS Testing Protocols ====== |
*[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] | *[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] | ||
*[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]] | *[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]] | ||
*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]] | *[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]] | ||
- | ====== | + | ====== 6. Antibiotic Resistance Testing ====== |
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*[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]] | *[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]] | ||
|}} | |}} | ||
<br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}} | <br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}} |
Latest revision as of 21:14, 29 October 2008
Experiments & Protocols
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