Team:University of Ottawa/19 July 2008

From 2008.igem.org

(Difference between revisions)
(Today in the lab)
Line 4: Line 4:
:'''Ligation'''
:'''Ligation'''
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
-
:''Ligation of Atcre'''
+
:'''Ligation of Atcre'''
::<li> Began ligation of Chris's Atcre component in ~50 uL reaction
::<li> Began ligation of Chris's Atcre component in ~50 uL reaction
 +
'''Matt'''
 +
:'''Inoculation'''
 +
::<li>Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
 +
::<li>I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
 +
:'''PCR'''
 +
::<li>I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.

Revision as of 14:58, 22 July 2008

Contents

Today in the lab

Dan

Ligation
  • Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
  • Ligation of Atcre
  • Began ligation of Chris's Atcre component in ~50 uL reaction
  • Matt

    Inoculation
  • Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
  • I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
  • PCR
  • I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.