Team:University of Ottawa/23 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: __TOC__ ==Today in the lab== '''Dan''' :'''PCR amplification of T123''' ::<li> I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow ::<li> 6...)
Line 7: Line 7:
:'''Transformation of p2S and p2D'''
:'''Transformation of p2S and p2D'''
::<li>p2S and p2D were transformed in E. coli
::<li>p2S and p2D were transformed in E. coli
 +
'''Chris'''
 +
:'''Minipreparation of AtCRE'''
 +
::<li> Used minprep kit and protocol to isolate AtCRE from inoculated cells.
 +
::<li> Measured absorbance of resulting DNA samples to determine concentrations
 +
:'''Confirmation of AtCRE'''
 +
::<li> Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
 +
::<li> Ran product on a 1% gel for 40 minutes at 80V
 +
::<li> Two of six samples showed desired bands.

Revision as of 15:32, 28 July 2008

Contents

Today in the lab

Dan

PCR amplification of T123
  • I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow
  • 6 tubes of PCR reaction were prepared for T123, hopefuly this will give me the amount of DNA that I need for a successful ligation.
    • Transformation of p2S and p2D
  • p2S and p2D were transformed in E. coli

    • Chris

    Minipreparation of AtCRE
  • Used minprep kit and protocol to isolate AtCRE from inoculated cells.
  • Measured absorbance of resulting DNA samples to determine concentrations
    • Confirmation of AtCRE
  • Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
  • Ran product on a 1% gel for 40 minutes at 80V
  • Two of six samples showed desired bands.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/23_July_2008"