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User:University of Washington/2 July 2008
From 2008.igem.org
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== BioBrick Promoter Measurements == | == BioBrick Promoter Measurements == | ||
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| + | - The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrigued at 14,000 RPM for 3 minutes, the supernatant was poured off, leaving only the pellet. | ||
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| + | - Pelleted cells were miniprepped using a Qiagen miniprep kit. | ||
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| + | - 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing. | ||
| + | |||
| + | - The remaining purified plasmid solutions were stored at -20 degrees Celsius. | ||
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[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 22:52, 2 July 2008
BioBrick Promoter Measurements
- The four overnight cultures of TOP10 cells containing promoter constructs I20260, I20268, I20269, and I20270 were centrigued at 14,000 RPM for 3 minutes, the supernatant was poured off, leaving only the pellet.
- Pelleted cells were miniprepped using a Qiagen miniprep kit.
- 4 uL of each plasmid sequence was added to its own two tubes. One tube had 8 uL of forward primer added to it, and the other had 8 uL of reverse primer added to it. These reaction tubes were submitted to the UW Sequencing Facility for sequencing.
- The remaining purified plasmid solutions were stored at -20 degrees Celsius.
Team:University_of_Washington/Notebook#Notebook
