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User:University of Washington/8 July 2008
From 2008.igem.org
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- VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water. | - VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water. | ||
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- 8 uL of diluted VF2 primer (1pmol/uL) was added to Eppendorf tubes. | - 8 uL of diluted VF2 primer (1pmol/uL) was added to Eppendorf tubes. | ||
| + | |||
- 4 uL of miniprepped plasmid DNA for parts I20260, I20268, I20269, and I20270 were added to tubes of primer, after appropriately labeling the Eppendorf tubes. | - 4 uL of miniprepped plasmid DNA for parts I20260, I20268, I20269, and I20270 were added to tubes of primer, after appropriately labeling the Eppendorf tubes. | ||
| + | |||
- Plasmid template and VF2 primer solutions were supplied to the UW DNA Sequencing Facility for reaction and analysis. | - Plasmid template and VF2 primer solutions were supplied to the UW DNA Sequencing Facility for reaction and analysis. | ||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 17:55, 8 July 2008
BioBrick Promoter Measurements
- VF2 primer (100pmol/uL) was diluted 1 to 100 with distilled water.
- 8 uL of diluted VF2 primer (1pmol/uL) was added to Eppendorf tubes.
- 4 uL of miniprepped plasmid DNA for parts I20260, I20268, I20269, and I20270 were added to tubes of primer, after appropriately labeling the Eppendorf tubes.
- Plasmid template and VF2 primer solutions were supplied to the UW DNA Sequencing Facility for reaction and analysis.
Back to Team:University_of_Washington/Notebook#Notebook
