"
User:University of Washington/14 July 2008
From 2008.igem.org
(→LuxR from AraC and TetR) |
|||
| Line 4: | Line 4: | ||
- A colony were selected from each section of the two plates (12 total) to prepare the overnight cultures. | - A colony were selected from each section of the two plates (12 total) to prepare the overnight cultures. | ||
| + | |||
| + | |||
| + | == BioBrick Promoter Measurements == | ||
| + | |||
| + | - Glycerol stocks of the TOP10 transformed with I20260, I20268, I20269, I20270, and the empty control cells were plated on four kanamycin plates, and one plate without antibiotic, respectively. | ||
| + | |||
| + | - Filter paper for the following parts were removed from the Registry binder, soaked in TE buffer, then centrifuged at 15,000 rpm for 3 minutes: I20259, I20261, I20270, E0240, P1010, J23100, J23100, J23103, J23104, J23106, J23107, J23108, J23109, J23110, J23111, J23112, J23114, J23115, J23117, J23118, J23119. | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 23:09, 14 July 2008
LuxR from AraC and TetR
- Got some single colonies from the old plates(from Friday). New plates had too many cells in them, no good single colonies.
- A colony were selected from each section of the two plates (12 total) to prepare the overnight cultures.
BioBrick Promoter Measurements
- Glycerol stocks of the TOP10 transformed with I20260, I20268, I20269, I20270, and the empty control cells were plated on four kanamycin plates, and one plate without antibiotic, respectively.
- Filter paper for the following parts were removed from the Registry binder, soaked in TE buffer, then centrifuged at 15,000 rpm for 3 minutes: I20259, I20261, I20270, E0240, P1010, J23100, J23100, J23103, J23104, J23106, J23107, J23108, J23109, J23110, J23111, J23112, J23114, J23115, J23117, J23118, J23119.
Back to Team:University_of_Washington/Notebook#Notebook
