Team:Rice University/Notebook/5 June 2008

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(Thursday 5 June)
(Thursday 5 June)
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*Taylor Stevenson
*Taylor Stevenson
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**XL1-Blue MR cells were prepared for phage infection as specified in the [[https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf]] and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.   
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**XL1-Blue MR cells were prepared for phage infection as specified in the [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf] and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.   
**'''Result'''-approximately 100 possibly lysogenic colonies grew on plate.
**'''Result'''-approximately 100 possibly lysogenic colonies grew on plate.
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Revision as of 20:37, 16 July 2008

Thursday 5 June

  • Selim Sheikh:
    • Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
20080604 gel.jpg

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)










  • Taylor Stevenson
    • XL1-Blue MR cells were prepared for phage infection as specified in the [1] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N.
    • Result-approximately 100 possibly lysogenic colonies grew on plate.





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