Team:Rice University/Notebook/17 July 2008

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*#30 cycles
*#30 cycles
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(1kb standard, BleoC PCR product, Stf PCR product)
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Latest revision as of 21:04, 19 July 2008

Thursday, July 17th

  • David Ouyang
    • Digest miniprep amber suppressor
    • ligate in front R0040
    • transform!
  • Taylor Stevenson
  • Primers for lambda phage genomic modification have finally arrived (NOTE: to view primers download this link and change extension to .doc (word file)[1])
  • PCR conditions
    1. 1uL of template (miniprepped BleoC in pSBA13, and NEB lambda DNA)
    2. annealing temp = 55*C
    3. extension time = 3min
    4. 30 cycles

20080717.jpg (1kb standard, BleoC PCR product, Stf PCR product)



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