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Team:The University of Alberta/25 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
*Sequenced TetR-RBS. Results yet to come. | *Sequenced TetR-RBS. Results yet to come. | ||
| - | *Digested PCR product of TetR-RBS with Xba/Pst. | + | *Digested PCR product of TetR-RBS with Xba/Pst. Gel Purified this part. |
| + | **(Re)ligated gel purified TetR.RBS to LacIQERE. | ||
*Transformed the ligation of J6-Tryp. | *Transformed the ligation of J6-Tryp. | ||
*Transformed LacIQERE-TetR.RBS. | *Transformed LacIQERE-TetR.RBS. | ||
*tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA | *tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA | ||
Revision as of 20:15, 25 July 2008
- Sequenced TetR-RBS. Results yet to come.
- Digested PCR product of TetR-RBS with Xba/Pst. Gel Purified this part.
- (Re)ligated gel purified TetR.RBS to LacIQERE.
- Transformed the ligation of J6-Tryp.
- Transformed LacIQERE-TetR.RBS.
- tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA
