Team:The University of Alberta/25 July 2008

From 2008.igem.org

(Difference between revisions)
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*Sequenced TetR-RBS. Results yet to come.
*Sequenced TetR-RBS. Results yet to come.
-
*Digested PCR product of TetR-RBS with Xba/Pst.
+
*Digested PCR product of TetR-RBS with Xba/Pst. Gel Purified this part.
 +
**(Re)ligated gel purified TetR.RBS to LacIQERE.
*Transformed the ligation of J6-Tryp.
*Transformed the ligation of J6-Tryp.
*Transformed LacIQERE-TetR.RBS.
*Transformed LacIQERE-TetR.RBS.
*tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA
*tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA

Revision as of 20:15, 25 July 2008

  • Sequenced TetR-RBS. Results yet to come.
  • Digested PCR product of TetR-RBS with Xba/Pst. Gel Purified this part.
    • (Re)ligated gel purified TetR.RBS to LacIQERE.
  • Transformed the ligation of J6-Tryp.
  • Transformed LacIQERE-TetR.RBS.
  • tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA