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| - | ='''Today in the Lab'''=
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| - | '''Tammy'''
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| - |
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| - | <TABLE BORDER=2>
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| - | <TR> <TD>'''Sample'''</TD><TD>'''Concentration (ng/μL)'''</TD><TD>'''V (μL)'''</TD><TD>'''Total DNA (ng)'''</TD> </TR>
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| - | <TR> <TD>pSSA14</TD><TD>129</TD><TD>7</TD><TD>903</TD> </TR>
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| - | <TR> <TD>OB</TD><TD>170</TD><TD>6</TD><TD>1020</TD> </TR>
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| - | </TABLE>
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| - |
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| - |
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| - | :<li> '''Digestion Step 1 of pSSA14 with PstI'''
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| - | <TABLE BORDER=2>
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| - | <TR> <TD>'''Digestion Reaction components'''</TD><TD>'''Vol (μl)'''</TD> </TR>
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| - | <TR> <TD>H2O</TD><TD>4</TD> </TR>
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| - | <TR> <TD>Buffer 3</TD><TD>1.5</TD> </TR>
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| - | <TR> <TD>BSA</TD><TD>1.5</TD> </TR>
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| - | <TR> <TD>PstI</TD><TD>1</TD> </TR>
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| - | <TR> <TD>DNA template</TD><TD>7</TD> </TR>
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| - | <TR> <TD>'''Total'''</TD><TD>'''15'''</TD> </TR>
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| - | </TABLE>
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| - |
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| - |
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| - | :<li> '''Digestion Step 1 of OB with PstI'''
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| - | <TABLE BORDER=2>
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| - | <TR> <TD>'''Digestion Reaction components'''</TD><TD>'''Vol (μl)'''</TD> </TR>
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| - | <TR> <TD>H2O</TD><TD>5</TD> </TR>
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| - | <TR> <TD>Buffer 3</TD><TD>1.5</TD> </TR>
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| - | <TR> <TD>BSA</TD><TD>1.5</TD> </TR>
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| - | <TR> <TD>PstI</TD><TD>1</TD> </TR>
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| - | <TR> <TD>DNA template</TD><TD>6</TD> </TR>
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| - | <TR> <TD>'''Total'''</TD><TD>'''15'''</TD> </TR>
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| - | </TABLE>
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| - |
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| - |
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| - | :<li>'''Digestion Step 2 of pSSA14 with ClaI'''
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| - | <TABLE BORDER=2>
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| - | <TR> <TD>'''Digestion Reaction components'''</TD><TD>'''Vol (μl)'''</TD> </TR>
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| - | <TR> <TD>H2O</TD><TD>9.5</TD> </TR>
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| - | <TR> <TD>Buffer 4</TD><TD>3</TD> </TR>
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| - | <TR> <TD>BSA</TD><TD>1.5</TD> </TR>
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| - | <TR> <TD>ClaI</TD><TD>1</TD> </TR>
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| - | <TR> <TD>DNA template</TD><TD>15</TD> </TR>
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| - | <TR> <TD>'''Total'''</TD><TD>'''30'''</TD> </TR>
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| - | </TABLE>
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| - |
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| - |
| |
| - | :<li>'''Digestion Step 2 of OB with ClaI'''
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| - | <TABLE BORDER=2>
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| - | <TR> <TD>'''Digestion Reaction components'''</TD><TD>'''Vol (μl)'''</TD> </TR>
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| - | <TR> <TD>H2O</TD><TD>9.5</TD> </TR>
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| - | <TR> <TD>Buffer 4</TD><TD>3</TD> </TR>
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| - | <TR> <TD>BSA</TD><TD>1.5</TD> </TR>
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| - | <TR> <TD>ClaI</TD><TD>1</TD> </TR>
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| - | <TR> <TD>DNA template</TD><TD>15</TD> </TR>
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| - | <TR> <TD>'''Total'''</TD><TD>'''30'''</TD> </TR>
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| - | </TABLE>
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"
