Team:University of Ottawa/28 July 2008

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(Today in the Lab)
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::<li> Control for PCR Reaction was ddH2O instead of DNA template
::<li> Control for PCR Reaction was ddH2O instead of DNA template
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::<li> 12 PCR Reactions with identical DNA template

Revision as of 20:07, 30 July 2008

Untitled Document

 

 


Today in the Lab

Tammy and Dan

SampleConcentration 1 (ng/μL)V1 (μL)Concentration 2 (ng/μL)V2 (μL)H2O (μL)
T12375456056


  • PCR Amplification of T123
  • PCR Reaction components1X Vol (μl)13X Vol (μL)
    5X Reaction Buffer10 130
    10 mM each dNTP113
    F Primer (10 pmol/μL) F692.532.5
    R Primer (10 pmol/μL) F702.532.5
    DNA Template452
    Phusion Polymerase0.56.5
    Filtered Sterile ddH2O29.5383.5
  • Control for PCR Reaction was ddH2O instead of DNA template
  • 12 PCR Reactions with identical DNA template