Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)

Revision as of 07:44, 12 August 2008

dock/undock dropdown

Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part	Plasmid	Description	µl left	ng/µl	Box	Date
[http://partsregistry.org/Part:BBa_M30109 M30109]	pSB1AC3	Light-sensor	+/- 40	50.4	1	July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]	pSB1AC3	Light-sensor	+/- 30	54.3	2	July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]	BBa_J61002 (Ap)	Constitutive promoter	+/- 60	345.0	3	July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]	BBa_J61002 (Ap)	Constitutive promoter	+/- 30	176.1	4	July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	RBS (efficiency 1)	+/- 20	73.8	5	July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	RBS (efficiency 1)	+/- 30	95.1	6	July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	RBS (efficiency 1)	+/- 40	104.0	7	July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]	pSB1A2	TetR repressor	+/- 25	177.1	8	July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]	pSB1A2	TetR repressor	+/- 30	161.7	9	July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 40	63.0	10	July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 10	112.3	11	July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 20	130.3	12	July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 45	303.1	13	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 30	213.6	14	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 48	311.0	31	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 40	214.0	47	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 40	209.5	48	July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]	pSB1A2	OmpF promoter	+/- 40	32.0	15	July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]	pSB1A2	OmpF promoter	+/- 30	60.9	16	July 24
[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	RBS (efficiency 0.3)	+/- 10	114.8	18	July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]	pSB1A2	eCFP	+/- 40	84.2	19	July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]	pSB1A2	eCFP	+/- 40	86.4	20	July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]	pSB1A2	LacI	+/- 30	133.1	21	July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]	pSB1A2	LacI repressor	+/- 40	146.4	49	July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]	pSB1A2	LacI repressor	+/- 40	203.1	50	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	3d lock	+/- 35	117.4	23	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	3d lock	+/- 39.5	144.8	24	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	3d lock	+/- 48	129.1	28	July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]	BBa_J23006 (Ap)	3d key+ terminator	+/- 40	160.7	25	July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]	BBa_J23006 (Ap)	3d key+ terminator	+/- 46	308.3	29	July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]	BBa_J61002 (Ap)	Constitutive Promoter	+/- 46	375.1	26	July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	404	30	July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 42.5	208.2	27	July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]	pSB1AK3	RBS + LuxI + Terminator	+/- 30	104.0	22	July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]	pSB1AK3	RBS + LuxI + Terminator	+/- 35	170.8	45	July 29
[http://partsregistry.org/Part:BBa_I712022 I712022]	BBa_J52017 (Km, Ap)	T7 polymerase	+/- 40	394.1	35	July 31
[http://partsregistry.org/Part:BBa_R0062 R0062]	pSB1A2	LuxR regulated promoter	+/- 40	104.1	37	July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]	pSB1A2	LacI regulated promoter	+/- 45	64.0	43	July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]	pSB1A2	LacI regulated promoter	+/- 40	69.8	44	July 29
[http://partsregistry.org/Part:BBa_I12006 I12006]	pSB2K3	434 cI repressible promoter	+/- 48	25.8	51	August 08
[http://partsregistry.org/Part:BBa_I12006 I12006]	pSB2K3	434 cI repressible promoter	+/- 48	52.7	52	August 08
[http://partsregistry.org/Part:BBa_J23116 J23116]	BBa_J61002 (Ap)	Constitutive promoter	+/- 48	328.8	53	August 08
[http://partsregistry.org/Part:BBa_C0060 C0060]	pSB1A2	lactonase production	+/- 48	84.2	60	August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]	pSB1A2	LuxR repressor	+/- 48	76.2	61	August 11
[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	RBS(medium)	+/- 48	51.0	62	August 11
[http://partsregistry.org/Part:BBa_R0040 R0040]	pSB1A2	tetR repressible promoter	+/- 48	37.9	63	August 11
[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for Cell Death	+/- 48	119.1	38	August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for TetR generator	+/- 48	131.0	39	August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for TetR generator	+/- 48	133.2	40	August 06
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for Reset	+/- 48	118.5	41	August 06
[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for Cell Death	+/- 46	41.0	42	August 06
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for Cell Death	+/- 46	124.8	54	August 08
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	GFP with LVA tag	+/- 46	152.7	55	August 08
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Output	+/- 46	17.5	56	August 08
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	89.9	57	August 08
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	94.4	58	August 08
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for Output	+/- 46	151.8	59	August 08

Restriction Digests
Part	Enzyme 1	Enzyme 2	vector/insert	length (bp)	µl left	ng/µl	Box	Date
[http://partsregistry.org/Part:BBa_J23100 J23100]	EcoR I	Spe I	Insert	52	38.1	2.6	1	July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]	EcoR I	Spe I	Insert	677	25	7.1	2	July 24
[http://partsregistry.org/Part:BBa_R0084 R0084]	EcoR I	Spe I	Insert	125	33.4	3.8	3	July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]	EcoR I	Spe I	Insert	779	22	9.5	4	July 24
[http://partsregistry.org/Part:BBa_J23109 J23109]	EcoR I	Spe I	Insert	52	21	4.2	5	July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]	EcoR I	Spe I	Insert	125	25	6.1	6	July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]	EcoR I	Spe I	Insert	62	23	3.4	7	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3090	2	12.9	8	July 30
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3090	45	1.3	9	July 31
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3308	25	5.1	22	August 07
[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3303	31	11.7	11	July 31
[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3303	21	13.4	20	August 07
[http://partsregistry.org/Part:BBa_R0011 R0011]	EcoR I	Spe I	Insert	72	20	8.5	12	July 30
[http://partsregistry.org/Part:BBa_R0011 R0011]	EcoR I	Spe I	Insert	72	20	7.5	13	July 30
[http://partsregistry.org/Part:BBa_C0062 C0062]	EcoR I	Spe I	Insert	774	18	24.7	14	August 01
[http://partsregistry.org/Part:BBa_C0060 C0060]	EcoR I	Spe I	Insert	806	15	8.2	15	August 01
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2]	EcoR I	Spe I	Plasmid	2079	17	18.2	16	August 01
[http://partsregistry.org/Part:BBa_P1010 P1010]	EcoR I	Spe I	Insert	682	10	4.9	17	August 01
[http://partsregistry.org/Part:BBa_K145015 K145015] (GFP-LVA)	EcoR I	Spe I	Insert	625	13	6.3	18	August 05
[http://partsregistry.org/Part:BBa_C0012 C0012]	EcoR I	Spe I	Insert	1145	12	5.6	19	August 05
[http://partsregistry.org/Part:BBa_J23022 J23022]	EcoR I	Xba I	Vector	3498	20	15.2	21	August 07
[http://partsregistry.org/Part:BBa_R0062 R0062]	EcoR I	Spe I	Insert	55	27	7.8	23	August 07
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3305	+/-25	5.1	22	August 07

Things stored in freezer in lab (-20°C)

Miscellaneous
Description	Name	µl left	Concentration	Box	Date
Primer GFP-LVA	1084	90	20 µM	1	August 01
Primer GFP-LVA	1085	90	20 µM	2	August 01
dNTP mix	ATP, GTP, TTP, CTP	70	10 mM	3	August 01
Primer GFP-LVA	1084	500	100 µM	4	August 01
Primer GFP-LVA	1085	500	100 µM	5	August 01
Primer Test	1074	800	100 µM	6	August 07
Primer Test	1075	780	100 µM	7	August 07
Primer T7-UmuD	1076	560	100 µM	8	August 07
Primer T7-UmuD	1077	477	100 µM	9	August 07
Primer T7-UmuD	1078	467	100 µM	10	August 07
Primer T7-UmuD	1079	45	100 µM	11	August 07
Primer Transduction	1097	627	100 µM	12	August 07
Primer Transduction	1098	710	100 µM	13	August 07
Primer Transduction	1099	608	100 µM	14	August 07
Primer Anti-sense LuxI	1100	522	100 µM	15	August 07
Primer Anti-sense LuxI	1101	237	100 µM	16	August 07
Primer Test	1074	100	20 µM	17	August 07
Primer Test	1075	100	20 µM	18	August 07
Primer T7-UmuD	1076	100	20 µM	19	August 07
Primer T7-UmuD	1077	100	20 µM	20	August 07
Primer T7-UmuD	1078	100	20 µM	21	August 07
Primer T7-UmuD	1079	100	20 µM	22	August 07
Primer Transduction	1097	100	20 µM	23	August 07
Primer Transduction	1098	100	20 µM	24	August 07
Primer Transduction	1099	100	20 µM	25	August 07
Primer Anti-sense LuxI	1100	100	20 µM	26	August 07
Primer Anti-sense LuxI	1101	100	20 µM	27	August 07
dNTP mix	ATP, GTP, TTP, CTP	90	2 mM	28	August 07

@@ Line 263: / Line 263: @@
 |- style="background: #E0FFFF; text-align: center;"
 |dNTP mix||ATP, GTP, TTP, CTP||90||2 mM||28||August 07
-|}
-==Ligations: step 1==
-{|
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Inputs
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-|J23116+B0032||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-|C0040+B0015||ok||ok||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-|R0040+E0240||-||-||-||-||-
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Output
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-| R0084+B0032||ok||ok||ok||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| E0022+B0015||ok||ok||ok||ok||ok
-|- style="background: #E0FFFF; text-align: center;"
-| K145015+B0015||pcr||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| E0040+B0015||-||-||-||-||-
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Memory
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-| R0053+P0152 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| R1052+P0353 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| R0084+B0033 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| C0056+B0015 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| R1052+K145013 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| K145013+B0015 ||-||-||-||-||-
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Filter
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-| R0084+J23022 ||ok||ok||ok||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| J230109+J23032 ||ok||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| K145014+B0015 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| K145001+B0015 ||-||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| I7122074+J23032 ||ok||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| IK0145015+B0015 ||pcr||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| E0040+B0015 ||-||-||-||-||-
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Inverter
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-| I712074+J23032||ok||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| C0012+B0015||ok||ok||ok||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| R0011+F1610||ok||-||-||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| R0011+E0240||-||-||-||-||-
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Reset
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-| I712074+J023022||ok||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| C0012+B0015||-||ok||ok||ok||-
-|- style="background: #003E81;color:white;width=100%"
-! colspan="6" style="background: #003E81" | Cell Death
-|- style="background: #99CCFF; text-align: center; width=100%"
-|width=200| '''Name'''
-|width=160| '''Miniprep'''
-|width=150| '''Digest'''
-|width=150| '''Ligation'''
-|width=150| '''Colonies'''
-|width=150| '''Confirmed'''
-|- style="background: #E0FFFF; text-align: center;"
-| J23100+B0032||ok||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| C0062+B0015||-||ok||ok||ok||ok
-|- style="background: #E0FFFF; text-align: center;"
-| R0062+B0032||ok||ok||ok||-||-
-|- style="background: #E0FFFF; text-align: center;"
-| P1010+B0015||-||ok||ok||ok||-
-|- style="background: #E0FFFF; text-align: center;"
-| R0062+E0240||-||-||-||-||-
 |}