User:University of Washington/15 August 2008

From 2008.igem.org

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-got colonies of transformation of LuxR/GFP + promoter circuit. Next is to overnight(Sunday?) and sequence(Monday?)
-got colonies of transformation of LuxR/GFP + promoter circuit. Next is to overnight(Sunday?) and sequence(Monday?)
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==Yeast Shuttle Plasmid==
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- Heat inactivated the restriction digest reactions for J63005 (vector) and J33202 (insert) at 80 degrees Celsius for 20 minutes.
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- Ran a gel with the vector and insert fragments, and circular and linearized pAC88.
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- Gel purified the vector and insert fragments using Qiagen Gel Purification kit.
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Revision as of 23:23, 15 August 2008

MG1655Z1(Faifan)

-streaked culture from glycerol stock on LB plate and incubated at 42 degree Celsius(Ingrid's lab).

LuxR from AraC and TetR(Faifan)

-got colonies of transformation of LuxR/GFP + promoter circuit. Next is to overnight(Sunday?) and sequence(Monday?)

Yeast Shuttle Plasmid

- Heat inactivated the restriction digest reactions for J63005 (vector) and J33202 (insert) at 80 degrees Celsius for 20 minutes.

- Ran a gel with the vector and insert fragments, and circular and linearized pAC88.

- Gel purified the vector and insert fragments using Qiagen Gel Purification kit.



Back to Team:University_of_Washington/Notebook#Notebook