"
Team:KULeuven/Project/CellDeath
From 2008.igem.org
(Difference between revisions)
(→Components) |
|||
| Line 9: | Line 9: | ||
===Components=== | ===Components=== | ||
| - | The luxR protein [http://partsregistry.org/Part:BBa_C0062 '''BBa_C0062'''] is placed under control of | + | The luxR protein [http://partsregistry.org/Part:BBa_C0062 '''BBa_C0062'''] is placed under control of a P22 CII hybrid promoter [http://partsregistry.org/Part:BBa_K145150 '''BBa_145150''']. The ''ccdB'' suicide gene [http://partsregistry.org/Part:BBa_P1010 '''BBa_P1010'''], on its part, is placed under control of the Luxp<sub>R</sub> promoter [http://partsregistry.org/Part:BBa_R0062 '''BBa_R0062''']. |
===Action=== | ===Action=== | ||
Revision as of 12:05, 27 August 2008
dock/undock dropdown
Contents |
Cell Death
BioBricks
Components
The luxR protein [http://partsregistry.org/Part:BBa_C0062 BBa_C0062] is placed under control of a P22 CII hybrid promoter [http://partsregistry.org/Part:BBa_K145150 BBa_145150]. The ccdB suicide gene [http://partsregistry.org/Part:BBa_P1010 BBa_P1010], on its part, is placed under control of the LuxpR promoter [http://partsregistry.org/Part:BBa_R0062 BBa_R0062].
Action
When the concentration of HSL is big enough (see the modeling page), HSL will form a complex with the LuxR protein and thus activate the LuxpR promoter. This results in ccdB production and eventually in cell death ([http://www.ncbi.nlm.nih.gov/pubmed/10196173?dopt=Abstract reference]).
Input
Output
Filter
InverTimer
Reset
Cell Death
Memory
