Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(Parts stored in the fridge in the lab (4°C))
(Parts stored in the fridge in the lab (4°C))
Line 294: Line 294:
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||13||July 30
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||13||July 30
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_E0240 E0240]|| ''Eco''R I||''Xba'' I||Vector||2955||28||25.2||53||September 1
+
|[http://partsregistry.org/Part:BBa_E0240 E0240]|| ''Eco''R I||''Xba'' I||Vector||2955||28||25.2||42||September 1
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_E0040 E0040]|| ''Eco''R I||''Spe'' I||Insert||720||28||8.3||37||September 1
|[http://partsregistry.org/Part:BBa_E0040 E0040]|| ''Eco''R I||''Spe'' I||Insert||720||28||8.3||37||September 1

Revision as of 09:36, 3 September 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

MiniPrep Products: Parts
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.37July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.38July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40303.19July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20213.610July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 48311.011July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30214.012July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40209.513July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.06July 23
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS(medium)+/- 551.057August 11
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS(medium)+/- 2047.255August 27
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS(medium)+/- 4027.056August 27
[http://partsregistry.org/Part:BBa_B0033 B0033]pSB1AK3RBS+/- 5036.543August 27
[http://partsregistry.org/Part:BBa_B0033 B0033]pSB1AK3RBS+/- 2338.444August 27
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.847July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.148July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.049July 29
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1AK3RBS+/- 155750August 27
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1AK3RBS+/- 5029.651August 27
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.120July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40146.422July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40203.121July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 20177.14July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.75July 24
[http://partsregistry.org/Part:BBa_C0060 C0060]pSB1A2lactonase production+/- 4884.253August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]pSB1A2LuxR repressor+/- 4876.238August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]pSB1AK3LuxR repressor+/- 2541.536August 27
[http://partsregistry.org/Part:BBa_C0062 C0062]pSB1AK3LuxR repressor+/- 5031.737August 27
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3HSL Sender Device+/- 5057.616August 12
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3HSL Sender Device+/- 4053.117August 12
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3HSL Sender Device+/- 5041.418August 27
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3HSL Sender Device+/- 2031.519August 27
[http://partsregistry.org/Part:BBa_I712022 I712022]BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.232July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 4520.533August 27
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 4563.934August 27
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 40160.726July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 46308.327July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 35117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 48129.125July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 46375.128July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4899.230August 27
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4869.231August 27
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4840429July 29
[http://partsregistry.org/Part:BBa_J23116 J23116]BBa_J61002 (Ap)Constitutive promoter +/- 48328.852August 08
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter+/- 4564.041July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter +/- 4069.842July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1AK3LacI regulated promoter+/- 5030.840August 27
[http://partsregistry.org/Part:BBa_R0040 R0040]pSB1A2tetR repressible promoter+/- 4837.946August 11
[http://partsregistry.org/Part:BBa_R0062 R0062]pSB1A2LuxR regulated promoter +/- 40104.13July 31
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.014July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.915July 24
[http://partsregistry.org/Part:BBa_C0060 C0060]pSB1A2Lactonase30155.354July 31
[http://partsregistry.org/Part:BBa_C0062 C0062]pSB1A2LuxR repressor/activator?159.435July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2lacI + pL promoter?68.039July 31
[http://partsregistry.org/Part:BBa_J23066 J23066]J23006ribokey+/- 4554158August 30
[http://partsregistry.org/Part:BBa_P0353 P0353]pSB1AK3cII p22 van memory+/- 45406.91August 30
[http://partsregistry.org/Part:BBa_P0152 P0152]pSB1A2cI 434 van memory+/- 44265.42August 30
[http://partsregistry.org/Part:BBa_E0240 E0240]pSB1A2GFP generator+/- 4428159August 30
[http://partsregistry.org/Part:BBa_K145015 K145015]pSB1A2GFP+LVA+/- 4825760August 30
[http://partsregistry.org/Part:BBa_K145151 K145151]pSB1A2ccdB+/- 48204.661August 30
[http://partsregistry.org/Part:BBa_K145001 K145001]pSB1A2T7 without UmuD+/- 48414.863August 30
[http://partsregistry.org/Part:BBa_C0056 C0056]pSB1A2cI represor+/- 45419.864August 30
[http://partsregistry.org/Part:BBa_C0061 C0061]pSB1A2autoinducer ASL synthetase, LuxI+/- 45338.665August 30
[http://partsregistry.org/Part:BBa_B0014 B0014]pSB1AK3terminator+/- 45344.466August 30
[http://partsregistry.org/Part:BBa_E0040 E0040]pSB1A2GFP+/- 48305.867August 30
[http://partsregistry.org/Part:BBa_K145151 K145151]pSB1A2ccdB+/- 48188.462August 30
[http://partsregistry.org/Part:BBa_R0053 R0053]pSB1A2p22 cII regulated promotor+/- 2572.169August 30
[http://partsregistry.org/Part:BBa_I714074 I714074]pSB1A2ribokey+/- 4846968August 30
[http://partsregistry.org/Part:BBa_R0040 R0040]pSB1A2tetR repressible promotor+/- 2774.645September 1


MiniPrep Products: Ligations
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 46124.81AAugust 08
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Output+/- 46151.85AAugust 08
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 4689.93AAugust 08
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 4694.44AAugust 08
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Input+/- 40102.58AAugust 25
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Input+/- 3596.22BAugust 25
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2GFP with LVA tag+/- 46152.72AAugust 08
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2ccdB coding region+/- 4657.85BAugust 28
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2ccdB coding region+/- 4672.18BAugust 28
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2ccdB coding region+/- 4618.57BAugust 28
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2ccdB coding region+/- 4623.36BAugust 28
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for output+/- 3064.49AAugust 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Output+/- 3593.11BAugust 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)Ligation for Filter (old)+/- 3581.33BAugust 27
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)Ligation for Filter (old)+/- 3586.34BAugust 27
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter (old)+/- 20150.26AAugust 20
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter (old)+/- 2058.37AAugust 20


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_B0015 B0015] Pst IXba IInsert144104.63August 29
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33031111.71July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33031013.62August 26
[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR IXba IVector2092517.625August 19
[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR IXba IVector2092206.424August 29
[http://partsregistry.org/Part:BBa_B0033 B0033] EcoR IXba IVector20922010.931August 27
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert677187.16July 24
[http://partsregistry.org/Part:BBa_C0012 C0012] Spe IPst IVector31922516.432August 29
[http://partsregistry.org/Part:BBa_C0060 C0060] Spe IPst IVector28532516.318August 29
[http://partsregistry.org/Part:BBa_C0040 C0040] Spe IPst IVector27242519.27August 29
[http://partsregistry.org/Part:BBa_K145015 K145015] Spe IPst IVector28232518.833August 29
[http://partsregistry.org/Part:BBa_K145151 K145151] Spe IPst IVector23702514.534August 29
[http://partsregistry.org/Part:BBa_K145001 K145001] Spe IPst IVector4782206.240August 29
[http://partsregistry.org/Part:BBa_C0061 C0061] Spe IPst IVector26802017.539August 31
[http://partsregistry.org/Part:BBa_C0056 C0056] Spe IPst IVector27002019.036August 31
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert803285.417August 12
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert80698.216August 01
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert7741824.711August 01
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert774258.312August 27
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert774285.010August 12
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert779229.54July 24
[http://partsregistry.org/Part:BBa_F1610 F1610] EcoR IXba IVector3956264.530August 19
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090212.920July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090451.321July 31
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3308255.122August 07
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.65July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52214.229July 29
[http://partsregistry.org/Part:BBa_J23116 J23116] EcoR ISpe IInsert52+/-208.126August 12
[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly) EcoR ISpe IInsert2732+/-814.141August 12
[http://partsregistry.org/Part:BBa_K145001 K145001] EcoR ISpe IInsert2730+/-2124.324August 22
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] EcoR ISpe IPlasmid2079918.216August 01
[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoR ISpe IPlasmid20562427.743August 25
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72208.513July 30
[http://partsregistry.org/Part:BBa_E0240 E0240] EcoR IXba IVector29552825.242September 1
[http://partsregistry.org/Part:BBa_E0040 E0040] EcoR ISpe IInsert720288.337September 1
[http://partsregistry.org/Part:BBa_C0056 C0056] EcoR ISpe IInsert6362310.135September 1
[http://partsregistry.org/Part:BBa_C0061 C0061] EcoR ISpe IInsert618289.038September 1
[http://partsregistry.org/Part:BBa_P0152 P0152] EcoR IXba IVector29302823.257September 1
[http://partsregistry.org/Part:BBa_P0353 P0353] EcoR IXba IVector40002815.358September 1
[http://partsregistry.org/Part:BBa_J23066 J23066] EcoR IXbaIVector27002811.459September 1
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33032012.64September 1
[http://partsregistry.org/Part:BBa_B0034 B0034] EcoR IXba IVector20912815.661September 1
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72207.514July 30
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72252.115August 27
[http://partsregistry.org/Part:BBa_R0062 R0062] EcoR ISpe IInsert55277.823August 07
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.88July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125256.19July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert61253.428July 29
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector4080+/-3032.833August 14
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector4086+/-3014.819August 14
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert112+/-303.928August 14
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert101+/-304.029August 14
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3113+/-208.331August 14
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert71+/-282.541August 25
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert144+/-200.918August 22
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert144+/-302.919August 21
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert90+/-282.638August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR ISpe IInsert311+/-283.645August 27
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert144+/-282.442August 25
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR ISpe IInsert365+/-206.930August 14
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert432+/-304.634August 22
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert432+/-303.737August 22

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer GFP-LVA10849020 µM1August 01
Primer GFP-LVA10859020 µM2August 01
dNTP mixATP, GTP, TTP, CTP7010 mM3August 01
Primer GFP-LVA1084500100 µM4August 01
Primer GFP-LVA1085500100 µM5August 01
Primer Test1074800100 µM6August 07
Primer Test1075780100 µM7August 07
Primer T7-UmuD1076560100 µM8August 07
Primer T7-UmuD1077477100 µM9August 07
Primer T7-UmuD1078467100 µM10August 07
Primer T7-UmuD107945100 µM11August 07
Primer Transduction1097627100 µM12August 07
Primer Transduction1098710100 µM13August 07
Primer Transduction1099608100 µM14August 07
Primer Anti-sense LuxI1100522100 µM15August 07
Primer Anti-sense LuxI1101237100 µM16August 07
Primer Test107410020 µM17August 07
Primer Test107510020 µM18August 07
Primer T7-UmuD107610020 µM19August 07
Primer T7-UmuD107710020 µM20August 07
Primer T7-UmuD107810020 µM21August 07
Primer T7-UmuD107910020 µM22August 07
Primer Transduction109710020 µM23August 07
Primer Transduction109810020 µM24August 07
Primer Transduction109910020 µM25August 07
Primer Anti-sense LuxI110010020 µM26August 07
Primer Anti-sense LuxI110110020 µM27August 07
dNTP mixATP, GTP, TTP, CTP902 mM28August 19
Primer ccdB1127100100 µM29August 19
Primer ccdB1128100100 µM30August 19
Primer K1451501129100100 µM31August 19
Primer K1451501130100100 µM32August 19
Primer ccdB11279020 µM33August 19
Primer ccdB112898020 µM34August 19
Primer K14515011299901 µM35August 19
Primer K14515011309901 µM36August 19
dNTP mixATP, GTP, TTP, CTP9902 µM37August 19