Imperial College/21 August 2008
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Revision as of 16:33, 5 September 2008
21 August 2008Dry Lab
Wet LabCloning
No insert can be observed for the terminator, this insert is too small to be visualised but a short run on a gel will be used to check for the presence of this insert tomorrow. The mRFP - terminator insert is approximately 1kb and can be seen, similarily GFP - Terminator is approximately 1kb (also shown). AK3 does not appear to have an insert (which would be correct as the nromal insert is lethal to E.coli and there is a 600bp insert clearly visible from the CAT digest. All registry sequences appear to be the correct size, indicating that they are probably what they are meant to be. B.subtilis
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