Imperial College/7 September 2008
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*AmyE 5' and 3' integration sequences and Aad9 (Spectinomycin resistance gene) to be integrated into pSB1A2 or pSB1AK3 | *AmyE 5' and 3' integration sequences and Aad9 (Spectinomycin resistance gene) to be integrated into pSB1A2 or pSB1AK3 | ||
*GeneArt constructs 3, 6, 10 and 12 (EpsE gene and PgsiB-RBSspoVG in construct 3, LipA-Elastin and PgsiB-RBSgsiB in construct 6, P43-RBSspoVG and Pveg-RBSspoVG), along with all above biobricks will be transformed into Xl1-Blue ''E.coli'' | *GeneArt constructs 3, 6, 10 and 12 (EpsE gene and PgsiB-RBSspoVG in construct 3, LipA-Elastin and PgsiB-RBSgsiB in construct 6, P43-RBSspoVG and Pveg-RBSspoVG), along with all above biobricks will be transformed into Xl1-Blue ''E.coli'' | ||
- | + | *Depending on results, LacI PCR to be digested and purified ready for ligation into pSB1A2/pSB1AK3 | |
- | + | **Monday or Tuesday:PCR trial all genomicseqeunces to determineoptimal conditions with Taq | |
=====Tuesday===== | =====Tuesday===== |
Revision as of 21:55, 7 September 2008
7th September 2008WetlabCloningMonday
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