"
Team:University of Lethbridge/Notebook/GeneralLabOctober
From 2008.igem.org
m (→October 5, 2008) |
m (→October 5, 2008) |
||
| Line 25: | Line 25: | ||
-Restriction Digest the pLacI, RFP and TetR plasmids obtained today, as well as recutting the TetR plasmid from last time, since it appears as though it didn't cut at all, or only cut once. | -Restriction Digest the pLacI, RFP and TetR plasmids obtained today, as well as recutting the TetR plasmid from last time, since it appears as though it didn't cut at all, or only cut once. | ||
| + | |||
| + | |||
| + | [[Image:Oct5gel.jpg | 250 px]] | ||
Revision as of 18:06, 5 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
October 1, 2008
Roxanne
-Inactivated the Enzymes in the morning
-Ran a gel of the tested pLacI, along with the previously cut RFP and TetR in the afternoon on 1% Agarose Gel in TAE.
-the iGEM pLacI had a faint band, RFP looks good (half of it cut), TetR looks like it either didn't cut at all, or only cut once. Only used 2 uL for each sample, will try running with more.
October 4, 2008
Roxanne
-Reran the pLacI tests, RFP and TetR on a 1% Agarose Gel in TAE.
-Repicked pLacI x2, RFP and TetR colonies into LB+amp media since I've been having trouble with ligations. Brent suggested using lots of DNA in the ligation (<100 ng) to make ure that the ligation does in fact work this time.
October 5, 2008
Roxanne
-plasmid prepped the pLacI x2, RFP and TetR subcultures. Lost one of the pLacI cultures (cells wouldn't lyse). Had some left over pLacI - 2 culture, attempted to plasmid prep those cells.
-Ran a gel of the plasmid preps.
-Restriction Digest the pLacI, RFP and TetR plasmids obtained today, as well as recutting the TetR plasmid from last time, since it appears as though it didn't cut at all, or only cut once.
