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Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)
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__NOTOC__ | __NOTOC__ | ||
| - | == | + | ==design== |
| - | ===[[Team:Chiba/ | + | |
| + | ==Method== | ||
| + | #Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908). | ||
| + | #Inoculated them independently in liquid media. Incubated at 37°C 12h | ||
| + | #Mixed them. | ||
| + | #Incubated at 37°C. | ||
| + | #Measured intensity of green fluorescence at regular time intervals. | ||
| + | |||
| + | [[Team:Chiba/protocol/phenotype/timedelay|more details...]] | ||
| + | |||
===Reaction temparature:37°C=== | ===Reaction temparature:37°C=== | ||
*センダーの培養液：500μL、レシーバの培養液：500μL | *センダーの培養液：500μL、レシーバの培養液：500μL | ||
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[[Image:Chiba_talks_JW1908_30_RS1_01.gif|frame|left|Fig. <br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_JW1908_30_RS1_01.gif|frame|left|Fig. <br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | ||
[[Image:Chiba_talks_JW1908_30_RS1_02.gif|frame|left|Fig. <br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_JW1908_30_RS1_02.gif|frame|left|Fig. <br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | ||
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| + | <br clear=all> | ||
| + | [[Team:Chiba/Sender experiments#Experiment|>Back to Sender experiment and result]] | ||
Revision as of 10:34, 29 October 2008
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design
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C.
- Measured intensity of green fluorescence at regular time intervals.
Reaction temparature:37°C
- センダーの培養液：500μL、レシーバの培養液：500μL
Fig.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Reaction temparature:30°C
- センダーの培養液：500μL、レシーバの培養液：500μL
Fig.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
>Back to Sender experiment and result
