Revision as of 18:10, 25 June 2008

Thursday 5 June

Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)

Taylor Stevenson
- Titered the phage stocks obtained from packaging the NEB lambda genome see packaging manual.pdf
- Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.

@@ Line 9: / Line 9: @@
 <BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR>
 *Taylor Stevenson
-**Titered the phage stocks obtained from packaging the NEB lambda genome
+**Titered the phage stocks obtained from packaging the NEB lambda genome see [[packaging manual.pdf]]
-*#Grow VCS275 supA+ strain in Lambda media (LB supplemented with 0.2% w/v maltose and 10mM MgS04) @ 30*C O/N, shaking @ 250rpm.
+**'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated.  Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
-*#Pellet cells @ 500xg for 10min, and re-suspend in 2x volume of 10mM MgS04.  Aliquot into 200uL fractions.
-*#Add serial dilutions of phage in SM buffer see [[packaging manual.pdf]]