Team:Rice University/Notebook/7 June 2008

From 2008.igem.org

(Difference between revisions)
(Saturday 7 June)
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[[Image:20080607_gel.jpg|100px|left]]
[[Image:20080607_gel.jpg|100px|left]]
(from left to right, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI)
(from left to right, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI)
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<BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR>
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*Taylor Stevenson
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**Phage infected XL1-blue MR colonies cultured in 96 deep-well plate were spotted onto two EMB plates using a 2uL plate replicator.  Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic). 
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**'''Result'''-both plates showed no bacterial growth after 24h.
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<BR><BR><BR><BR>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Rice_University|Home]]
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!align="center"|[[Team:Rice_University/Team|The Team]]
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!align="center"|[[Team:Rice_University/Project|The Project]]
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!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Rice_University/Modeling|Modeling]]
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!align="center"|[[Team:Rice_University/Notebook|Notebook]]
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|}

Revision as of 19:13, 25 June 2008

Saturday 7 June

  • Selim Sheikh:
    • Prepared digestion of lambda DNA with XhoI only
    • Prepared gel electrophoresis of above digest along with solution of lambda DNA with 1Kb standard
20080607 gel.jpg

(from left to right, lane 1: 1Kb, lane 2: lambda DNA, lane 3: lambda with XhoI)
















  • Taylor Stevenson
    • Phage infected XL1-blue MR colonies cultured in 96 deep-well plate were spotted onto two EMB plates using a 2uL plate replicator. Both plates were incubated O/N, one at 30*C and one at 42*C (temp is high enough to denature the CI repressor, causing any lysogen to become lytic).
    • Result-both plates showed no bacterial growth after 24h.





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